Kruljac-Letunic A, Blaukat A (2005) Assay and functional properties of the tyrosine kinase Pyk2 in regulation of Arf1 through ASAP1 phosphorylation. Methods Enzymol 404, 411-22 16413287

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Y308-p - DDEF1 (mouse) ▲

Modsite: PKEVGGLyVASRANS SwissProt Entrez-Gene Orthologous residues DDEF1 (human): ‑, DDEF1 iso2 (human): ‑, DDEF1 (mouse): Y308‑p, DDEF1 (rat): Y308‑p, DDEF1 (cow): ‑ Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE Pyk2 (human) Downstream Regulation Effect of modification (function):  activity, inhibited, molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Disrupts far-Western Comments:  leads to less binding to phospholipids (including P(4,5)P2, PI(3,4,5)P3, and PI(3,4)P2) and less GAP activity toward Arf1

Y782-p - DDEF1 (mouse) ▲

Modsite: RDKQRLSyGAFTNQI SwissProt Entrez-Gene Orthologous residues DDEF1 (human): Y767‑p, DDEF1 iso2 (human): Y770‑p, DDEF1 (mouse): Y782‑p, DDEF1 (rat): Y779‑p, DDEF1 (cow): Y770‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE Pyk2 (human) Downstream Regulation Effect of modification (function):  activity, inhibited, molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Disrupts far-Western Comments:  leads to less binding to phospholipids (including P(4,5)P2, PI(3,4,5)P3, and PI(3,4)P2) and less GAP activity toward Arf1