Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 19450681
Deheuninck J, et al. (2009) Phosphorylation of the MET receptor on juxtamembrane tyrosine residue 1001 inhibits its caspase-dependent cleavage. Cell Signal 21, 1455-63 19450681
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S1000-p - Met (human)
Modsite: tEMVsNEsVDyRAtF SwissProt Entrez-Gene
Orthologous residues
Met (human): S1000‑p, Met iso2 (human): S1018‑p, Met (mouse): S998‑p, Met (rat): S1001‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical), MDCK (epithelial)
Cellular systems studied:  cell lines
Species studied:  dog, human

Y1003-p - Met (human)
Modsite: VsNEsVDyRAtFPED SwissProt Entrez-Gene
Orthologous residues
Met (human): Y1003‑p, Met iso2 (human): Y1021‑p, Met (mouse): Y1001‑p, Met (rat): Y1004‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical), MDCK (epithelial)
Cellular systems studied:  cell lines
Species studied:  dog, human
Downstream Regulation
Effect of modification (function):  molecular association, regulation, protein stabilization, ubiquitination
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CBL (human) Induces co-immunoprecipitation
Comments:  inhibits caspase-dependent cleavage

Y1234-p - Met (human)
Modsite: RDMyDkEyysVHNkt SwissProt Entrez-Gene
Orthologous residues
Met (human): Y1234‑p, Met iso2 (human): Y1252‑p, Met (mouse): Y1232‑p, Met (rat): Y1235‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
HGF increase
anisomycin HGF no effect upon treatment-induced increase
LY294002, U0126 HGF inhibit treatment-induced increase
LY294002, U0126 no change compared to control
Su11274 no change compared to control

Y1235-p - Met (human)
Modsite: DMyDkEyysVHNktG SwissProt Entrez-Gene
Orthologous residues
Met (human): Y1235‑p, Met iso2 (human): Y1253‑p, Met (mouse): Y1233‑p, Met (rat): Y1236‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
HGF increase
anisomycin HGF no effect upon treatment-induced increase
LY294002, U0126 HGF inhibit treatment-induced increase
LY294002, U0126 no change compared to control
Su11274 no change compared to control