Horváth D, et al. (2017) Myosin phosphatase and RhoA-activated kinase modulate neurotransmitter release by regulating SNAP-25 of SNARE complex. PLoS One 12, e0177046 28486561

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T138-p - SNAP-25 (mouse) ▲

Modsite: GGFIRRVtNDARENE SwissProt Entrez-Gene Orthologous residues SNAP‑25 (human): T138‑p, SNAP‑25 iso2 (human): T138‑p, SNAP‑25 (mouse): T138‑p, SNAP‑25 (rat): T138‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, phospho-antibody, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  B50 (neural crest) Cellular systems studied:  cell lines Species studied:  rat Enzymes shown to modify site in vitro:  Type Enzyme KINASE ROCK2 (human) PHOSPHATASE PPP1CA (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE ROCK2 (rat) co-immunoprecipitation, pharmacological inhibitor of upstream enzyme, phospho-antibody, phosphopeptide analysis PHOSPHATASE MYPT1 (rat) siRNA inhibition of enzyme, co-immunoprecipitation, phospho-antibody Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  exocytosis, inhibited Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays STX1A (mouse) Disrupts co-immunoprecipitation