Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 28479321
Zhang Q, et al. (2017) Ska3 Phosphorylated by Cdk1 Binds Ndc80 and Recruits Ska to Kinetochores to Promote Mitotic Progression. Curr Biol 27, 1477-1484.e4 28479321
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

T358-p - SKA3 (human)
Modsite: SYENLLRtPtPPEVT SwissProt Entrez-Gene
Orthologous residues
SKA3 (human): T358‑p, SKA3 iso3 (human): T358‑p, SKA3 (mouse): T363‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  E.coli (bacterial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK1 (human) phosphopeptide analysis, pharmacological inhibitor of upstream enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  cell cycle regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HEC1 (human) Induces pull-down assay
Comments:  kinetochore localization

T360-p - SKA3 (human)
Modsite: ENLLRtPtPPEVTkI SwissProt Entrez-Gene
Orthologous residues
SKA3 (human): T360‑p, SKA3 iso3 (human): T360‑p, SKA3 (mouse): S365‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  E.coli (bacterial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK1 (human) phosphopeptide analysis, pharmacological inhibitor of upstream enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  cell cycle regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HEC1 (human) Induces pull-down assay
Comments:  kinetochore localization