Curated Information
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Home > Curated Information Page > PubMed Id: 28472170
Hinz L, et al. (2017) Two serines in the distal C-terminus of the human ß1-adrenoceptor determine ß-arrestin2 recruitment. PLoS One 12, e0176450 28472170
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S260-p - ADRB1 (human)
Modsite: KQVKKIDsCERRFLG SwissProt Entrez-Gene
Orthologous residues
ADRB1 (human): S260‑p, ADRB1 (mouse): S260‑p, ADRB1 (rat): S260‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S274-p - ADRB1 (human)
Modsite: GGPARPPsPSPsPVP SwissProt Entrez-Gene
Orthologous residues
ADRB1 (human): S274‑p, ADRB1 (mouse): S274‑p, ADRB1 (rat): S274‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
norepinephrine increase

S278-p - ADRB1 (human)
Modsite: RPPsPSPsPVPAPAP SwissProt Entrez-Gene
Orthologous residues
ADRB1 (human): S278‑p, ADRB1 (mouse): , ADRB1 (rat):
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S312-p - ADRB1 (human)
Modsite: RAGKRRPsRLVALRE SwissProt Entrez-Gene
Orthologous residues
ADRB1 (human): S312‑p, ADRB1 (mouse): S301‑p, ADRB1 (rat): S301‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

T404-p - ADRB1 (human)
Modsite: AARRRHAtHGDRPRA SwissProt Entrez-Gene
Orthologous residues
ADRB1 (human): T404‑p, ADRB1 (mouse): A393‑p, ADRB1 (rat): A393‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S412-p - ADRB1 (human)
Modsite: HGDRPRAsGCLARPG SwissProt Entrez-Gene
Orthologous residues
ADRB1 (human): S412‑p, ADRB1 (mouse): S401‑p, ADRB1 (rat): S401‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
norepinephrine increase

S423-p - ADRB1 (human)
Modsite: ARPGPPPsPGAAsDD SwissProt Entrez-Gene
Orthologous residues
ADRB1 (human): S423‑p, ADRB1 (mouse): S412‑p, ADRB1 (rat): S412‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S461-p - ADRB1 (human)
Modsite: GAAADSDssLDEPCR SwissProt Entrez-Gene
Orthologous residues
ADRB1 (human): S461‑p, ADRB1 (mouse): S450‑p, ADRB1 (rat): S450‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
norepinephrine increase
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation, receptor internalization, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ARRB1 (human) Induces FRET
Comments:  beta-arrestin recruitment to the ADRB1

S462-p - ADRB1 (human)
Modsite: AAADSDssLDEPCRP SwissProt Entrez-Gene
Orthologous residues
ADRB1 (human): S462‑p, ADRB1 (mouse): S451‑p, ADRB1 (rat): S451‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
norepinephrine increase
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation, receptor internalization, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ARRB1 (human) Induces FRET
Comments:  beta-arrestin recruitment to the ADRB1