Curated Information
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Home > Curated Information Page > PubMed Id: 16760428
Qi W, Tang Z, Yu H (2006) Phosphorylation- and polo-box-dependent binding of Plk1 to Bub1 is required for the kinetochore localization of Plk1. Mol Biol Cell 17, 3705-16 16760428
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S593-p - BUB1 (human)
Modsite: CNktLAPsPksPGDF SwissProt Entrez-Gene
Orthologous residues
BUB1 (human): S593‑p, BUB1 (mouse): S579‑p
Characterization
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

T609-p - BUB1 (human)
Modsite: sAAQLAstPFHkLPV SwissProt Entrez-Gene
Orthologous residues
BUB1 (human): T609‑p, BUB1 (mouse): T595‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PLK1 (human) Induces intracellular localization co-immunoprecipitation