Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 16760434
Le Boeuf F, Houle F, Sussman M, Huot J (2006) Phosphorylation of focal adhesion kinase (FAK) on Ser732 is induced by rho-dependent kinase and is essential for proline-rich tyrosine kinase-2-mediated phosphorylation of FAK on Tyr407 in response to vascular endothelial growth factor. Mol Biol Cell 17, 3508-20 16760434
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

Y397-p - FAK (human)
Modsite: sVsEtDDyAEIIDEE SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y397‑p, FAK iso2 (human): Y216‑p, FAK iso5 (human): Y397‑p, FAK (mouse): Y397‑p, FAK iso2 (mouse): Y428‑p, FAK iso4 (mouse): Y397‑p, FAK iso9 (mouse): , FAK (rat): Y397‑p, FAK (chicken): Y397‑p, FAK iso5 (chicken):
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HUVEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF increase

Y407-p - FAK (human)
Modsite: IIDEEDtytMPSTRD SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y407‑p, FAK iso2 (human): Y226‑p, FAK iso5 (human): Y407‑p, FAK (mouse): Y407‑p, FAK iso2 (mouse): Y438‑p, FAK iso4 (mouse): Y407‑p, FAK iso9 (mouse): , FAK (rat): Y407‑p, FAK (chicken): Y407‑p, FAK iso5 (chicken):
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HUVEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Pyk2 (human) antisense inhibition of upstream enzyme, activation of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF increase

S732-p - FAK (human)
Modsite: SsEGFYPsPQHMVQT SwissProt Entrez-Gene
Orthologous residues
FAK (human): S732‑p, FAK iso2 (human): S580‑p, FAK iso5 (human): S732‑p, FAK (mouse): S732‑p, FAK iso2 (mouse): S763‑p, FAK iso4 (mouse): S732‑p, FAK iso9 (mouse): S40‑p, FAK (rat): S732‑p, FAK (chicken): S732‑p, FAK iso5 (chicken): S40‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  HUVEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  S732A mutant impaired the phosphorylation of Y407 in response to VEGF
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ROCK1 (human) antisense inhibition of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF increase
VEGF, Y27632 inhibit treatment-induced increase
geldanamycin, VEGF inhibit treatment-induced increase

S843-p - FAK (human)
Modsite: DVRLsRGsIDREDGs SwissProt Entrez-Gene
Orthologous residues
FAK (human): S843‑p, FAK iso2 (human): , FAK iso5 (human): S843‑p, FAK (mouse): S843‑p, FAK iso2 (mouse): S874‑p, FAK iso4 (mouse): S843‑p, FAK iso9 (mouse): S151‑p, FAK (rat): S843‑p, FAK (chicken): S845‑p, FAK iso5 (chicken): S151‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HUVEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF increase
VEGF, Y27632 no effect upon treatment-induced increase
geldanamycin, VEGF no effect upon treatment-induced increase

Y402-p - Pyk2 (human)
Modsite: CsIEsDIyAEIPDEt SwissProt Entrez-Gene
Orthologous residues
Pyk2 (human): Y402‑p, Pyk2 iso2 (human): Y402‑p, Pyk2 (mouse): Y402‑p, Pyk2 (rat): Y402‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  HUVEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced