Williams BL, et al. (1999) Phosphorylation of Tyr319 in ZAP-70 is required for T-cell antigen receptor-dependent phospholipase C-gamma1 and Ras activation. EMBO J 18, 1832-44 10202147

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Y319-p - ZAP70 (human) ▲

Modsite: tsVyEsPysDPEELk SwissProt Entrez-Gene Orthologous residues ZAP70 (human): Y319‑p, ZAP70 (mouse): Y318‑p, ZAP70 (rat): Y314‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  COS (fibroblast), Jurkat (T lymphocyte), P116 (T lymphocyte) Cellular systems studied:  cell lines Species studied:  green monkey, human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes antibody increase Downstream Regulation Effect of modification (function):  activity, induced, molecular association, regulation, phosphorylation Effect of modification (process):  transcription, altered Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Lck (human) SH2 Induces activity, induced pull-down assay PLCG1 (human) SH2 Induces activity, induced pull-down assay Comments:  Ca(2+) mobilization