Luo J, et al. (2004) Acetylation of p53 augments its site-specific DNA binding both in vitro and in vivo. Proc Natl Acad Sci U S A 101, 2259-64 14982997

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K370-ac - p53 (human) ▲

Modsite: RAHsSHLkskkGQst SwissProt Entrez-Gene Orthologous residues p53 (human): K370‑ac, p53 iso2 (human): ‑, p53 iso4 (human): K331‑ac, p53 (mouse): K367‑ac, p53 iso2 (mouse): ‑, p53 (rat): K368‑ac, p53 (rabbit): K368‑ac, p53 (green monkey): K370‑ac Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell large cell lung carcinoma Relevant cell lines - cell types - tissues:  E.coli (bacterial), NCI-H1299 (pulmonary), NCI-H460 (pulmonary) Cellular systems studied:  cell lines Species studied:  bacteria, human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes etoposide increase trichostatin_A increase nicotinamide increase Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces EMSA, in vitro, co-immunoprecipitation

K372-ac - p53 (human) ▲

Modsite: HsSHLkskkGQstsR SwissProt Entrez-Gene Orthologous residues p53 (human): K372‑ac, p53 iso2 (human): ‑, p53 iso4 (human): K333‑ac, p53 (mouse): K369‑ac, p53 iso2 (mouse): ‑, p53 (rat): K370‑ac, p53 (rabbit): K370‑ac, p53 (green monkey): K372‑ac Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell large cell lung carcinoma Relevant cell lines - cell types - tissues:  E.coli (bacterial), NCI-H1299 (pulmonary), NCI-H460 (pulmonary) Cellular systems studied:  cell lines Species studied:  bacteria, human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes etoposide increase trichostatin_A increase nicotinamide increase Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces EMSA, in vitro, co-immunoprecipitation

K373-ac - p53 (human) ▲

Modsite: sSHLkskkGQstsRH SwissProt Entrez-Gene Orthologous residues p53 (human): K373‑ac, p53 iso2 (human): ‑, p53 iso4 (human): K334‑ac, p53 (mouse): K370‑ac, p53 iso2 (mouse): ‑, p53 (rat): K371‑ac, p53 (rabbit): K371‑ac, p53 (green monkey): K373‑ac Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell large cell lung carcinoma Relevant cell lines - cell types - tissues:  E.coli (bacterial), NCI-H1299 (pulmonary), NCI-H460 (pulmonary) Cellular systems studied:  cell lines Species studied:  bacteria, human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes etoposide increase trichostatin_A increase nicotinamide increase Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces EMSA, in vitro, co-immunoprecipitation

K381-ac - p53 (human) ▲

Modsite: GQstsRHkkLMFktE SwissProt Entrez-Gene Orthologous residues p53 (human): K381‑ac, p53 iso2 (human): ‑, p53 iso4 (human): K342‑ac, p53 (mouse): K378‑ac, p53 iso2 (mouse): ‑, p53 (rat): K379‑ac, p53 (rabbit): K379‑ac, p53 (green monkey): K381‑ac Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell large cell lung carcinoma Relevant cell lines - cell types - tissues:  E.coli (bacterial), NCI-H1299 (pulmonary), NCI-H460 (pulmonary) Cellular systems studied:  cell lines Species studied:  bacteria, human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes etoposide increase trichostatin_A increase nicotinamide increase Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces EMSA, in vitro, co-immunoprecipitation

K382-ac - p53 (human) ▲

Modsite: QstsRHkkLMFktEG SwissProt Entrez-Gene Orthologous residues p53 (human): K382‑ac, p53 iso2 (human): ‑, p53 iso4 (human): K343‑ac, p53 (mouse): K379‑ac, p53 iso2 (mouse): ‑, p53 (rat): K380‑ac, p53 (rabbit): K380‑ac, p53 (green monkey): K382‑ac Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell large cell lung carcinoma Relevant cell lines - cell types - tissues:  E.coli (bacterial), NCI-H1299 (pulmonary), NCI-H460 (pulmonary) Cellular systems studied:  cell lines Species studied:  bacteria, human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes etoposide increase trichostatin_A increase nicotinamide increase Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces EMSA, in vitro, co-immunoprecipitation