Curated Information
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Home > Curated Information Page > PubMed Id: 11250899
Ito A, et al. (2001) p300/CBP-mediated p53 acetylation is commonly induced by p53-activating agents and inhibited by MDM2. EMBO J 20, 1331-40 11250899
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K320-ac - p53 (human)
Modsite: sssPQPkkkPLDGEy SwissProt Entrez-Gene
Orthologous residues
p53 (human): K320‑ac, p53 iso2 (human): K320‑ac, p53 iso4 (human): K281‑ac, p53 (mouse): K317‑ac, p53 iso2 (mouse): K317‑ac, p53 (rat): K318‑ac, p53 (rabbit): K318‑ac, p53 (green monkey): K320‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma
Relevant cell lines - cell types - tissues:  A549 (pulmonary), HEK293T (epithelial), NCI-H1299 (pulmonary), WI-38 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  protein stabilization

K370-ac - p53 (human)
Modsite: RAHsSHLkskkGQst SwissProt Entrez-Gene
Orthologous residues
p53 (human): K370‑ac, p53 iso2 (human): , p53 iso4 (human): K331‑ac, p53 (mouse): K367‑ac, p53 iso2 (mouse): , p53 (rat): K368‑ac, p53 (rabbit): K368‑ac, p53 (green monkey): K370‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma
Relevant cell lines - cell types - tissues:  A549 (pulmonary), HEK293T (epithelial), NCI-H1299 (pulmonary), WI-38 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  protein stabilization

K372-ac - p53 (human)
Modsite: HsSHLkskkGQstsR SwissProt Entrez-Gene
Orthologous residues
p53 (human): K372‑ac, p53 iso2 (human): , p53 iso4 (human): K333‑ac, p53 (mouse): K369‑ac, p53 iso2 (mouse): , p53 (rat): K370‑ac, p53 (rabbit): K370‑ac, p53 (green monkey): K372‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma
Relevant cell lines - cell types - tissues:  A549 (pulmonary), HEK293T (epithelial), NCI-H1299 (pulmonary), WI-38 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  protein stabilization

K373-ac - p53 (human)
Modsite: sSHLkskkGQstsRH SwissProt Entrez-Gene
Orthologous residues
p53 (human): K373‑ac, p53 iso2 (human): , p53 iso4 (human): K334‑ac, p53 (mouse): K370‑ac, p53 iso2 (mouse): , p53 (rat): K371‑ac, p53 (rabbit): K371‑ac, p53 (green monkey): K373‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma
Relevant cell lines - cell types - tissues:  A549 (pulmonary), HEK293T (epithelial), NCI-H1299 (pulmonary), WI-38 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  protein stabilization

K381-ac - p53 (human)
Modsite: GQstsRHkkLMFktE SwissProt Entrez-Gene
Orthologous residues
p53 (human): K381‑ac, p53 iso2 (human): , p53 iso4 (human): K342‑ac, p53 (mouse): K378‑ac, p53 iso2 (mouse): , p53 (rat): K379‑ac, p53 (rabbit): K379‑ac, p53 (green monkey): K381‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma
Relevant cell lines - cell types - tissues:  A549 (pulmonary), HEK293T (epithelial), NCI-H1299 (pulmonary), WI-38 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  protein stabilization

K382-ac - p53 (human)
Modsite: QstsRHkkLMFktEG SwissProt Entrez-Gene
Orthologous residues
p53 (human): K382‑ac, p53 iso2 (human): , p53 iso4 (human): K343‑ac, p53 (mouse): K379‑ac, p53 iso2 (mouse): , p53 (rat): K380‑ac, p53 (rabbit): K380‑ac, p53 (green monkey): K382‑ac
Characterization
Methods used to characterize site in vivo modification-specific antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma
Relevant cell lines - cell types - tissues:  A549 (pulmonary), HEK293T (epithelial), NCI-H1299 (pulmonary), WI-38 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
ACETYLTRANSFERASE p300 (human) transfection of inactive enzyme, transfection of wild-type enzyme
ACETYLTRANSFERASE CBP (human) inhibition of upstream enzyme, transfection of inactive enzyme, transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UVB increase
deferoxamine increase
H2O2 increase
MG115 increase
PALA increase
actinomycin_D increase
MG115 no change compared to control
leptomycin_B increase
PCAF (human) no change compared to control
MDM2 (human) decrease
trichostatin_A MDM2 (human) inhibit treatment-induced decrease
IL23A (human) MDM2 (human) inhibit treatment-induced decrease
Downstream Regulation
Effect of modification (function):  protein stabilization