Curated Information
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Home > Curated Information Page > PubMed Id: 16549426
Li X, et al. (2006) Autophosphorylation of Akt at threonine 72 and serine 246. A potential mechanism of regulation of Akt kinase activity. J Biol Chem 281, 13837-43 16549426
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T72-p - Akt1 (human)
Modsite: tERPRPNtFIIRCLQ SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T72‑p, Akt1 iso2 (human): T10‑p, Akt1 (mouse): T72‑p, Akt1 (rat): T72‑p, Akt1 (fruit fly): T175‑p, Akt1 (cow): T72‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Comments:  requires phosphorylation of sites S473 and T308
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  apoptosis, inhibited
Comments:  doxorubicin-induced apoptosis

S246-p - Akt1 (human)
Modsite: LSRERVFsEDRARFY SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S246‑p, Akt1 iso2 (human): S184‑p, Akt1 (mouse): S246‑p, Akt1 (rat): S246‑p, Akt1 (fruit fly): T362‑p, Akt1 (cow): S246‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Comments:  requires phosphorylation of sites S473 and T308
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  apoptosis, inhibited
Comments:  doxorubicin-induced apoptosis