Samuelsson AM, et al. (2006) Hyperinsulinemia: effect on cardiac mass/function, angiotensin II receptor expression, and insulin signaling pathways. Am J Physiol Heart Circ Physiol 291, H787-96 16565309

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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T308-p - Akt1 (rat) ▲

Modsite: KDGATMKtFCGTPEy SwissProt Entrez-Gene Orthologous residues Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocardium Cellular systems studied:  tissue Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase adrenalectomy insulin no effect upon treatment-induced increase possible slight inhibition metoprolol insulin no effect upon treatment-induced increase possible slight augmentation

S473-p - Akt1 (rat) ▲

Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene Orthologous residues Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocardium Cellular systems studied:  tissue Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase only slight increase adrenalectomy insulin inhibit treatment-induced increase metoprolol insulin inhibit treatment-induced increase

T309-p - Akt2 (rat) ▲

Modsite: SDGATMKtFCGTPEY SwissProt Entrez-Gene Orthologous residues Akt2 (human): T309‑p, Akt2 (mouse): T309‑p, Akt2 (rat): T309‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocardium Cellular systems studied:  tissue Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase adrenalectomy insulin no effect upon treatment-induced increase possible slight inhibition metoprolol insulin no effect upon treatment-induced increase possible slight augmentation

S474-p - Akt2 (rat) ▲

Modsite: RTHFPQFsYSASIRE SwissProt Entrez-Gene Orthologous residues Akt2 (human): S474‑p, Akt2 (mouse): S474‑p, Akt2 (rat): S474‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocardium Cellular systems studied:  tissue Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase only slight increase adrenalectomy insulin inhibit treatment-induced increase metoprolol insulin inhibit treatment-induced increase

T305-p - Akt3 (rat) ▲

Modsite: TDAATMKtFCGTPEY SwissProt Entrez-Gene Orthologous residues Akt3 (human): T305‑p, Akt3 (mouse): T305‑p, Akt3 (rat): T305‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocardium Cellular systems studied:  tissue Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase adrenalectomy insulin no effect upon treatment-induced increase possible slight inhibition metoprolol insulin no effect upon treatment-induced increase possible slight augmentation

T203-p - ERK1 (rat) ▲

Modsite: HDHTGFLtEyVAtRW SwissProt Entrez-Gene Orthologous residues ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocardium Cellular systems studied:  tissue Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase adrenalectomy insulin inhibit treatment-induced increase metoprolol insulin inhibit treatment-induced increase

Y205-p - ERK1 (rat) ▲

Modsite: HTGFLtEyVAtRWYR SwissProt Entrez-Gene Orthologous residues ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocardium Cellular systems studied:  tissue Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase adrenalectomy insulin inhibit treatment-induced increase metoprolol insulin inhibit treatment-induced increase

T183-p - ERK2 (rat) ▲

Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene Orthologous residues ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocardium Cellular systems studied:  tissue Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase adrenalectomy insulin inhibit treatment-induced increase metoprolol insulin inhibit treatment-induced increase

Y185-p - ERK2 (rat) ▲

Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene Orthologous residues ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocardium Cellular systems studied:  tissue Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase adrenalectomy insulin inhibit treatment-induced increase metoprolol insulin inhibit treatment-induced increase

S369-p - IRS1 (rat) ▲

Modsite: PPLNHSRsIPMPSSR SwissProt Entrez-Gene Orthologous residues IRS1 (human): S374‑p, IRS1 (mouse): S369‑p, IRS1 (rat): S369‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocardium Cellular systems studied:  tissue Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase adrenalectomy insulin inhibit treatment-induced increase metoprolol insulin no effect upon treatment-induced increase possible slight inhibition

Y987-p - IRS1 (rat) ▲

Modsite: VPNSRGDyMTMQIGC SwissProt Entrez-Gene Orthologous residues IRS1 (human): Y989‑p, IRS1 (mouse): Y983‑p, IRS1 (rat): Y987‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocardium Cellular systems studied:  tissue Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin decrease adrenalectomy insulin inhibit treatment-induced decrease metoprolol insulin inhibit treatment-induced increase

S212-p - MEK1 (rat) ▲

Modsite: KLCDFGVsGQLIDsM SwissProt Entrez-Gene Orthologous residues MEK1 (human): S212‑p, MEK1 iso2 (human): S186‑p, MEK1 (mouse): S212‑p, MEK1 (rat): S212‑p, MEK1 (rabbit): S212‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocardium Cellular systems studied:  tissue Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase adrenalectomy insulin inhibit treatment-induced increase metoprolol insulin inhibit treatment-induced increase

S218-p - MEK1 (rat) ▲

Modsite: VsGQLIDsMANsFVG SwissProt Entrez-Gene Orthologous residues MEK1 (human): S218‑p, MEK1 iso2 (human): S192‑p, MEK1 (mouse): S218‑p, MEK1 (rat): S218‑p, MEK1 (rabbit): S218‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocardium Cellular systems studied:  tissue Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase adrenalectomy insulin inhibit treatment-induced increase metoprolol insulin inhibit treatment-induced increase

S222-p - MEK2 (rat) ▲

Modsite: VSGQLIDsMANsFVG SwissProt Entrez-Gene Orthologous residues MEK2 (human): S222‑p, MEK2 (mouse): S222‑p, MEK2 (rat): S222‑p, MEK2 (chicken): S220‑p, MEK2 (cow): S222‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocardium Cellular systems studied:  tissue Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase adrenalectomy insulin inhibit treatment-induced increase metoprolol insulin inhibit treatment-induced increase

S226-p - MEK2 (rat) ▲

Modsite: LIDsMANsFVGTRSY SwissProt Entrez-Gene Orthologous residues MEK2 (human): S226‑p, MEK2 (mouse): S226‑p, MEK2 (rat): S226‑p, MEK2 (chicken): S224‑p, MEK2 (cow): S226‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocardium Cellular systems studied:  tissue Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase adrenalectomy insulin inhibit treatment-induced increase metoprolol insulin inhibit treatment-induced increase

T412-p - p70S6K (rat) ▲

Modsite: NQVFLGFtYVAPSVL SwissProt Entrez-Gene Orthologous residues p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocardium Cellular systems studied:  tissue Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase adrenalectomy insulin augment treatment-induced increase metoprolol insulin augment treatment-induced increase

T444-p - p70S6K (rat) ▲

Modsite: RFIGsPRtPVsPVKF SwissProt Entrez-Gene Orthologous residues p70S6K (human): T444‑p, p70S6K iso2 (human): T421‑p, p70S6K (mouse): T444‑p, p70S6K (rat): T444‑p, p70S6K iso2 (rat): T421‑p, p70S6K (fruit fly): ‑ Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocardium Cellular systems studied:  tissue Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase adrenalectomy insulin no effect upon treatment-induced increase metoprolol insulin inhibit treatment-induced increase

S447-p - p70S6K (rat) ▲

Modsite: GsPRtPVsPVKFsPG SwissProt Entrez-Gene Orthologous residues p70S6K (human): S447‑p, p70S6K iso2 (human): S424‑p, p70S6K (mouse): S447‑p, p70S6K (rat): S447‑p, p70S6K iso2 (rat): S424‑p, p70S6K (fruit fly): ‑ Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocardium Cellular systems studied:  tissue Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase adrenalectomy insulin no effect upon treatment-induced increase metoprolol insulin inhibit treatment-induced increase

Y508-p - PIK3R1 (rat) ▲

Modsite: QERYSKDyIEKFKRE SwissProt Entrez-Gene Orthologous residues PIK3R1 (human): Y508‑p, PIK3R1 iso2 (human): Y238‑p, PIK3R1 iso4 (human): Y508‑p, PIK3R1 (mouse): Y508‑p, PIK3R1 (rat): Y508‑p, PIK3R1 (cow): Y508‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocardium Cellular systems studied:  tissue Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase adrenalectomy insulin inhibit treatment-induced increase metoprolol insulin inhibit treatment-induced increase