Dioum EM, et al. (2009) Regulation of hypoxia-inducible factor 2alpha signaling by the stress-responsive deacetylase sirtuin 1. Science 324, 1289-93 19498162

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K385-ac - HIF2A (human) ▲

Modsite: GKGAVsEkSNFLFTk SwissProt Entrez-Gene Orthologous residues HIF2A (human): K385‑ac, HIF2A (mouse): K385‑ac, HIF2A (rat): K385‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), Hep3B (hepatic) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes DEACETYLASE SIRT1 (human) genetic knockout/knockin of upstream enzyme, transfection of inactive enzyme, pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme, pharmacological activator of upstream enzyme, transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes hypoxia increase Downstream Regulation Effect of modification (process):  transcription, inhibited

K685-ac - HIF2A (human) ▲

Modsite: TFKTRSAkGFGARGP SwissProt Entrez-Gene Orthologous residues HIF2A (human): K685‑ac, HIF2A (mouse): K689‑ac, HIF2A (rat): K689‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), Hep3B (hepatic) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes DEACETYLASE SIRT1 (human) genetic knockout/knockin of upstream enzyme, transfection of inactive enzyme, pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme, pharmacological activator of upstream enzyme, transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes hypoxia increase Downstream Regulation Effect of modification (process):  transcription, inhibited

K741-ac - HIF2A (human) ▲

Modsite: HLMWKRMkNLRGGSC SwissProt Entrez-Gene Orthologous residues HIF2A (human): K741‑ac, HIF2A (mouse): K745‑ac, HIF2A (rat): K745‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), Hep3B (hepatic) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes DEACETYLASE SIRT1 (human) genetic knockout/knockin of upstream enzyme, transfection of inactive enzyme, pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme, pharmacological activator of upstream enzyme, transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes hypoxia increase Downstream Regulation Effect of modification (process):  transcription, inhibited