Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.6
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 29959233
Kam CY, et al. (2018) Desmoplakin maintains gap junctions by inhibiting Ras/MAPK and lysosomal degradation of connexin-43. J Cell Biol 29959233
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S279-p - GJA1 (mouse)
Modsite: SsPtAPLsPMsPPGy SwissProt Entrez-Gene
Orthologous residues
GJA1 (human): S279‑p, GJA1 (mouse): S279‑p, GJA1 (rat): S279‑p, GJA1 (rabbit): S279‑p, GJA1 (pig): S279‑p, GJA1 (hamster): S279‑p, GJA1 (cow): S280‑p
Characterization
Methods used to characterize site in vivo immunoassay, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  epidermal, heart, myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  mouse, rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK2 (mouse) pharmacological inhibitor of upstream enzyme
KINASE ERK1 (mouse) pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Desmoplakin (mouse) decrease
U0126 Desmoplakin (mouse) augment treatment-induced decrease
Downstream Regulation
Effect of modification (function):  intracellular localization, protein degradation, receptor internalization, induced, ubiquitination

S282-p - GJA1 (mouse)
Modsite: tAPLsPMsPPGykLV SwissProt Entrez-Gene
Orthologous residues
GJA1 (human): S282‑p, GJA1 (mouse): S282‑p, GJA1 (rat): S282‑p, GJA1 (rabbit): S282‑p, GJA1 (pig): S282‑p, GJA1 (hamster): S282‑p, GJA1 (cow): S283‑p
Characterization
Methods used to characterize site in vivo immunoassay, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  epidermal, heart, myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  mouse, rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK2 (mouse) pharmacological inhibitor of upstream enzyme
KINASE ERK1 (mouse) pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Desmoplakin (mouse) decrease
U0126 Desmoplakin (mouse) augment treatment-induced decrease
Downstream Regulation
Effect of modification (function):  intracellular localization, protein degradation, receptor internalization, induced, ubiquitination