Curated Information
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Home > Curated Information Page > PubMed Id: 19377469
Tian C, et al. (2009) KRAB-type zinc-finger protein Apak specifically regulates p53-dependent apoptosis. Nat Cell Biol 11, 580-91 19377469
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K120-ac - p53 (human)
Modsite: FLhSGTAkSVTCTyS SwissProt Entrez-Gene
Orthologous residues
p53 (human): K120‑ac, p53 iso2 (human): K120‑ac, p53 iso4 (human): K81‑ac, p53 (mouse): K117‑ac, p53 iso2 (mouse): K117‑ac, p53 (rat): K118‑ac, p53 (rabbit): K117‑ac, p53 (green monkey): K120‑ac
Characterization
Methods used to characterize site in vivo mutation of modification site
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  K to R mutation of 6 or 8 sites
Downstream Regulation
Effect of modification (process):  transcription, altered
Comments:  ZNF420 (Apak) represses p53 activity by attenuating its acetylation

K164-ac - p53 (human)
Modsite: VRAMAIYkQSQHMTE SwissProt Entrez-Gene
Orthologous residues
p53 (human): K164‑ac, p53 iso2 (human): K164‑ac, p53 iso4 (human): K125‑ac, p53 (mouse): K161‑ac, p53 iso2 (mouse): K161‑ac, p53 (rat): K162‑ac, p53 (rabbit): K161‑ac, p53 (green monkey): K164‑ac
Characterization
Methods used to characterize site in vivo mutation of modification site
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  K to R mutation of 6 or 8 sites
Downstream Regulation
Effect of modification (process):  transcription, altered
Comments:  ZNF420 (Apak) represses p53 activity by attenuating its acetylation

K370-ac - p53 (human)
Modsite: RAHsSHLkskkGQst SwissProt Entrez-Gene
Orthologous residues
p53 (human): K370‑ac, p53 iso2 (human): , p53 iso4 (human): K331‑ac, p53 (mouse): K367‑ac, p53 iso2 (mouse): , p53 (rat): K368‑ac, p53 (rabbit): K368‑ac, p53 (green monkey): K370‑ac
Characterization
Methods used to characterize site in vivo mutation of modification site
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  K to R mutation of 6 or 8 sites
Downstream Regulation
Effect of modification (process):  transcription, altered
Comments:  ZNF420 (Apak) represses p53 activity by attenuating its acetylation

K372-ac - p53 (human)
Modsite: HsSHLkskkGQstsR SwissProt Entrez-Gene
Orthologous residues
p53 (human): K372‑ac, p53 iso2 (human): , p53 iso4 (human): K333‑ac, p53 (mouse): K369‑ac, p53 iso2 (mouse): , p53 (rat): K370‑ac, p53 (rabbit): K370‑ac, p53 (green monkey): K372‑ac
Characterization
Methods used to characterize site in vivo mutation of modification site
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  K to R mutation of 6 or 8 sites
Downstream Regulation
Effect of modification (process):  transcription, altered
Comments:  ZNF420 (Apak) represses p53 activity by attenuating its acetylation

K373-ac - p53 (human)
Modsite: sSHLkskkGQstsRH SwissProt Entrez-Gene
Orthologous residues
p53 (human): K373‑ac, p53 iso2 (human): , p53 iso4 (human): K334‑ac, p53 (mouse): K370‑ac, p53 iso2 (mouse): , p53 (rat): K371‑ac, p53 (rabbit): K371‑ac, p53 (green monkey): K373‑ac
Characterization
Methods used to characterize site in vivo mutation of modification site
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  K to R mutation of 6 or 8 sites
Downstream Regulation
Effect of modification (process):  transcription, altered
Comments:  ZNF420 (Apak) represses p53 activity by attenuating its acetylation

K381-ac - p53 (human)
Modsite: GQstsRHkkLMFktE SwissProt Entrez-Gene
Orthologous residues
p53 (human): K381‑ac, p53 iso2 (human): , p53 iso4 (human): K342‑ac, p53 (mouse): K378‑ac, p53 iso2 (mouse): , p53 (rat): K379‑ac, p53 (rabbit): K379‑ac, p53 (green monkey): K381‑ac
Characterization
Methods used to characterize site in vivo mutation of modification site
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  K to R mutation of 6 or 8 sites
Downstream Regulation
Effect of modification (process):  transcription, altered
Comments:  ZNF420 (Apak) represses p53 activity by attenuating its acetylation

K382-ac - p53 (human)
Modsite: QstsRHkkLMFktEG SwissProt Entrez-Gene
Orthologous residues
p53 (human): K382‑ac, p53 iso2 (human): , p53 iso4 (human): K343‑ac, p53 (mouse): K379‑ac, p53 iso2 (mouse): , p53 (rat): K380‑ac, p53 (rabbit): K380‑ac, p53 (green monkey): K382‑ac
Characterization
Methods used to characterize site in vivo mutation of modification site
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  K to R mutation of 6 or 8 sites
Downstream Regulation
Effect of modification (process):  transcription, altered
Comments:  ZNF420 (Apak) represses p53 activity by attenuating its acetylation

K386-ac - p53 (human)
Modsite: RHkkLMFktEGPDsD SwissProt Entrez-Gene
Orthologous residues
p53 (human): K386‑ac, p53 iso2 (human): , p53 iso4 (human): K347‑ac, p53 (mouse): K383‑ac, p53 iso2 (mouse): , p53 (rat): K384‑ac, p53 (rabbit): K384‑ac, p53 (green monkey): K386‑ac
Characterization
Methods used to characterize site in vivo mutation of modification site
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  K to R mutation of 6 or 8 sites
Downstream Regulation
Effect of modification (process):  transcription, altered
Comments:  ZNF420 (Apak) represses p53 activity by attenuating its acetylation

S68-p - ZNF420 (human)
Modsite: NTYETELsQWEMSDR SwissProt Entrez-Gene
Orthologous residues
ZNF420 (human): S68‑p, ZNF420 (mouse):
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  bone cancer
Relevant cell lines - cell types - tissues:  U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) genetic knockout/knockin of upstream enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MMS increase
siRNA MMS inhibit treatment-induced increase ATM siRNA
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
p53 (human) Disrupts enzymatic activity, inhibited apoptosis, altered co-immunoprecipitation
Comments:  in response to DNA damage, ZNF420 (Apak) dissociates from p53 and activates p53-mediated apoptosis