Zhang Y, et al. (2007) Dynamic receptor-dependent activation of inducible nitric-oxide synthase by ERK-mediated phosphorylation of Ser745. J Biol Chem 282, 32453-61 17804409

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S745-p - iNOS (human) ▲

Modsite: KSRQNLQsPTSSRAT SwissProt Entrez-Gene Orthologous residues iNOS (human): S745‑p, iNOS (mouse): S739‑p, iNOS (rat): S742‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), HMVEC (endothelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE ERK1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE ERK1 (human) pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme, co-immunoprecipitation, microscopy-colocalization, phospho-antibody Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes DAKD increase PD98059 DAKD inhibit treatment-induced increase Downstream Regulation Effect of modification (function):  enzymatic activity, induced