Curated Information
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Home > Curated Information Page > PubMed Id: 19289471
Sinnett-Smith J, et al. (2009) Protein Kinase D Mediates Mitogenic Signaling by Gq-coupled Receptors through Protein Kinase C-independent Regulation of Activation Loop Ser744 and Ser748 Phosphorylation. J Biol Chem 284, 13434-45 19289471
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T203-p - ERK1 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bombesin increase
GF109203X bombesin inhibit treatment-induced increase
vasopressin increase
GF109203X vasopressin inhibit treatment-induced increase
AG1478 vasopressin inhibit treatment-induced increase
bombesin increase
AG1478 bombesin inhibit treatment-induced increase
compound_56 bombesin inhibit treatment-induced increase

Y205-p - ERK1 (mouse)
Modsite: HtGFLtEyVAtRWyR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bombesin increase
GF109203X bombesin inhibit treatment-induced increase
vasopressin increase
GF109203X vasopressin inhibit treatment-induced increase
AG1478 vasopressin inhibit treatment-induced increase
bombesin increase
AG1478 bombesin inhibit treatment-induced increase
compound_56 bombesin inhibit treatment-induced increase

T183-p - ERK2 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  Swiss 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bombesin increase
GF109203X bombesin inhibit treatment-induced increase
vasopressin increase
GF109203X vasopressin inhibit treatment-induced increase
AG1478 vasopressin inhibit treatment-induced increase
bombesin increase
AG1478 bombesin inhibit treatment-induced increase
compound_56 bombesin inhibit treatment-induced increase

Y185-p - ERK2 (mouse)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  Swiss 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bombesin increase
GF109203X bombesin inhibit treatment-induced increase
vasopressin increase
GF109203X vasopressin inhibit treatment-induced increase
AG1478 vasopressin inhibit treatment-induced increase
bombesin increase
AG1478 bombesin inhibit treatment-induced increase
compound_56 bombesin inhibit treatment-induced increase

S941-p - FAK iso2 (mouse)
Modsite: KLQPQEIsPPPtANL SwissProt Entrez-Gene
Orthologous residues
FAK (human): S910‑p, FAK iso2 (human): S737‑p, FAK iso5 (human): S923‑p, FAK (mouse): S910‑p, FAK iso2 (mouse): S941‑p, FAK iso4 (mouse): S913‑p, FAK iso9 (mouse): S218‑p, FAK (rat): S913‑p, FAK (chicken): S911‑p, FAK iso5 (chicken): S217‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  Swiss 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bombesin increase
GF109203X bombesin inhibit treatment-induced increase

S152-p - MARCKS (mouse)
Modsite: kkKKKRFsFKKsFkL SwissProt Entrez-Gene
Orthologous residues
MARCKS (human): S159‑p, MARCKS (mouse): S152‑p, MARCKS (rat): S152‑p, MARCKS (chicken): S124‑p, MARCKS (cow): S158‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  Swiss 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bombesin increase
GF109203X bombesin inhibit treatment-induced increase

S156-p - MARCKS (mouse)
Modsite: KRFsFKKsFkLsGFs SwissProt Entrez-Gene
Orthologous residues
MARCKS (human): S163‑p, MARCKS (mouse): S156‑p, MARCKS (rat): S156‑p, MARCKS (chicken): S128‑p, MARCKS (cow): S162‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  Swiss 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bombesin increase
GF109203X bombesin inhibit treatment-induced increase

T562-p - p90RSK (mouse)
Modsite: AENGLLMtPCYtANF SwissProt Entrez-Gene
Orthologous residues
p90RSK (human): T573‑p, p90RSK iso2 (human): T582‑p, p90RSK (mouse): T562‑p, p90RSK iso3 (mouse): , p90RSK (rat): T573‑p, p90RSK (chicken): T590‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  Swiss 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bombesin increase
GF109203X bombesin inhibit treatment-induced increase

S744-p - PRKD1 (mouse)
Modsite: ARIIGEksFRRsVVG SwissProt Entrez-Gene
Orthologous residues
PRKD1 (human): S738‑p, PRKD1 (mouse): S744‑p, PRKD1 (rat): S744‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  Swiss 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bombesin increase
GF109203X bombesin inhibit treatment-induced increase
Go_6983 bombesin inhibit treatment-induced increase
vasopressin increase
GF109203X vasopressin inhibit treatment-induced increase
phorbol_ester increase
GF109203X phorbol_ester inhibit treatment-induced increase
PDGF increase
GF109203X PDGF inhibit treatment-induced increase
bombesin increase
K252a bombesin no effect upon treatment-induced increase

S748-p - PRKD1 (mouse)
Modsite: GEksFRRsVVGtPAy SwissProt Entrez-Gene
Orthologous residues
PRKD1 (human): S742‑p, PRKD1 (mouse): S748‑p, PRKD1 (rat): S748‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  Swiss 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PRKD1 (mouse)
Comments:  autophosphorylation
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bombesin increase
GF109203X bombesin inhibit treatment-induced increase
Go_6983 bombesin inhibit treatment-induced increase
vasopressin increase
GF109203X vasopressin inhibit treatment-induced increase
phorbol_ester increase
GF109203X phorbol_ester inhibit treatment-induced increase
PDGF increase
GF109203X PDGF inhibit treatment-induced increase
bombesin increase
K252a bombesin inhibit treatment-induced increase

S916-p - PRKD1 (mouse)
Modsite: KALSERVsIL_____ SwissProt Entrez-Gene
Orthologous residues
PRKD1 (human): S910‑p, PRKD1 (mouse): S916‑p, PRKD1 (rat): S916‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  Swiss 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bombesin increase
GF109203X bombesin inhibit treatment-induced increase
Go_6983 bombesin inhibit treatment-induced increase
vasopressin increase
GF109203X vasopressin inhibit treatment-induced increase
phorbol_ester increase
GF109203X phorbol_ester inhibit treatment-induced increase
PDGF increase
GF109203X PDGF inhibit treatment-induced increase
bombesin increase
K252a bombesin inhibit treatment-induced increase