Yamaguchi Y, et al. (2004) AML1 is functionally regulated through p300-mediated acetylation on specific lysine residues. J Biol Chem 279, 15630-8 14752096

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K24-ac - AML1 (human) ▲

Modsite: stALsPGkMSEALPL SwissProt Entrez-Gene Orthologous residues AML1 (human): K24‑ac, AML1 iso2 (human): K24‑ac, AML1 iso7 (human): K24‑ac, AML1 iso8 (human): K51‑ac, AML1 iso10 (human): K39‑ac, AML1 (mouse): K24‑ac, AML1 (rat): K24‑ac Characterization Methods used to characterize site in vivo:  3H acetate labeling, [14C] acetate labeling, immunoprecipitation, mutation of modification site, western blotting Disease tissue studied:  leukemia, T cell leukemia Relevant cell lines - cell types - tissues:  3T3 (fibroblast), COS7 (fibroblast), HEK293T (epithelial), HeLa (cervical), M1 (myeloid), MOLT-4 (T lymphocyte) Cellular systems studied:  cell lines Species studied:  bacteria, green monkey, human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  cell growth, induced, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces EMSA Comments:  transformation increased

K43-ac - AML1 (human) ▲

Modsite: AGAALAGkLRSGDRs SwissProt Entrez-Gene Orthologous residues AML1 (human): K43‑ac, AML1 iso2 (human): K43‑ac, AML1 iso7 (human): K43‑ac, AML1 iso8 (human): K70‑ac, AML1 iso10 (human): K58‑ac, AML1 (mouse): K43‑ac, AML1 (rat): K43‑ac Characterization Methods used to characterize site in vivo:  3H acetate labeling, [14C] acetate labeling, immunoprecipitation, mutation of modification site, western blotting Disease tissue studied:  leukemia, T cell leukemia Relevant cell lines - cell types - tissues:  3T3 (fibroblast), COS7 (fibroblast), HEK293T (epithelial), HeLa (cervical), M1 (myeloid), MOLT-4 (T lymphocyte) Cellular systems studied:  cell lines Species studied:  bacteria, green monkey, human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  cell growth, induced, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces EMSA Comments:  transformation increased