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Home > Curated Information Page > PubMed Id: 14752096
Yamaguchi Y, et al. (2004) AML1 is functionally regulated through p300-mediated acetylation on specific lysine residues. J Biol Chem 279, 15630-8 14752096
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K24-ac - AML1 (human)
Modsite: stALsPGkMSEALPL SwissProt Entrez-Gene
Orthologous residues
AML1 (human): K24‑ac, AML1 iso2 (human): K24‑ac, AML1 iso7 (human): K24‑ac, AML1 iso8 (human): K51‑ac, AML1 iso10 (human): K39‑ac, AML1 (mouse): K24‑ac, AML1 (rat): K24‑ac
Characterization
Methods used to characterize site in vivo 3H acetate labeling, [14C] acetate labeling, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), COS7 (fibroblast), HEK293T (epithelial), HeLa (cervical), M1 (myeloid), MOLT-4 (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  bacteria, green monkey, human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cell growth, induced, transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Induces EMSA
Comments:  transformation increased

K43-ac - AML1 (human)
Modsite: AGAALAGkLRSGDRs SwissProt Entrez-Gene
Orthologous residues
AML1 (human): K43‑ac, AML1 iso2 (human): K43‑ac, AML1 iso7 (human): K43‑ac, AML1 iso8 (human): K70‑ac, AML1 iso10 (human): K58‑ac, AML1 (mouse): K43‑ac, AML1 (rat): K43‑ac
Characterization
Methods used to characterize site in vivo 3H acetate labeling, [14C] acetate labeling, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), COS7 (fibroblast), HEK293T (epithelial), HeLa (cervical), M1 (myeloid), MOLT-4 (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  bacteria, green monkey, human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cell growth, induced, transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Induces EMSA
Comments:  transformation increased