Fukuoka M, et al. (2003) Negative regulation of forkhead transcription factor AFX (Foxo4) by CBP-induced acetylation. Int J Mol Med 12, 503-8 12964026

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K186-ac - FOXO4 (human) ▲

Modsite: MLNPEGGkSGkAPRR SwissProt Entrez-Gene Orthologous residues FOXO4 (human): K186‑ac, FOXO4 (mouse): K186‑ac, FOXO4 (rat): K186‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  liver cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HepG2 (hepatic) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE CBP (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes DEACETYLASE HDAC1 (human) pharmacological inhibitor of upstream enzyme ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, co-immunoprecipitation, transfection of inactive enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes trichostatin_A increase Downstream Regulation Effect of modification (function):  activity, inhibited Effect of modification (process):  transcription, inhibited

K189-ac - FOXO4 (human) ▲

Modsite: PEGGkSGkAPRRRAA SwissProt Entrez-Gene Orthologous residues FOXO4 (human): K189‑ac, FOXO4 (mouse): K189‑ac, FOXO4 (rat): K189‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  liver cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HepG2 (hepatic) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE CBP (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes DEACETYLASE HDAC1 (human) pharmacological inhibitor of upstream enzyme ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, co-immunoprecipitation, transfection of inactive enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes trichostatin_A increase Downstream Regulation Effect of modification (function):  activity, inhibited Effect of modification (process):  transcription, inhibited

K407-ac - FOXO4 (human) ▲

Modsite: GGLPSSSkLATGVGL SwissProt Entrez-Gene Orthologous residues FOXO4 (human): K407‑ac, FOXO4 (mouse): K408‑ac, FOXO4 (rat): K408‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  liver cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HepG2 (hepatic) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE CBP (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, co-immunoprecipitation, transfection of inactive enzyme DEACETYLASE HDAC1 (human) pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes trichostatin_A increase Downstream Regulation Effect of modification (function):  activity, inhibited Effect of modification (process):  transcription, inhibited