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Home > Curated Information Page > PubMed Id: 16012755
Kobayashi Y, et al. (2005) SIRT1 is critical regulator of FOXO-mediated transcription in response to oxidative stress. Int J Mol Med 16, 237-43 16012755
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K186-ac - FOXO4 (mouse)
Modsite: MLNPDGGkGGkAPRR SwissProt Entrez-Gene
Orthologous residues
FOXO4 (human): K186‑ac, FOXO4 (mouse): K186‑ac, FOXO4 (rat): K186‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial), HeLa (cervical), Jurkat (T lymphocyte), Saos-2 (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
DEACETYLASE SIRT1 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
ACETYLTRANSFERASE p300 (mouse) transfection of wild-type enzyme
DEACETYLASE SIRT1 (mouse) pharmacological activator of upstream enzyme, transfection of inactive enzyme, pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme, co-immunoprecipitation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nicotinamide increase
resveratrol decrease
H2O2 increase
LY294002 increase
Downstream Regulation
Effect of modification (process):  transcription, inhibited

K189-ac - FOXO4 (mouse)
Modsite: PDGGkGGkAPRRRAA SwissProt Entrez-Gene
Orthologous residues
FOXO4 (human): K189‑ac, FOXO4 (mouse): K189‑ac, FOXO4 (rat): K189‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial), HeLa (cervical), Jurkat (T lymphocyte), Saos-2 (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
DEACETYLASE SIRT1 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (mouse) pharmacological activator of upstream enzyme, transfection of inactive enzyme, pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme, co-immunoprecipitation
ACETYLTRANSFERASE p300 (mouse) transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nicotinamide increase
resveratrol decrease
H2O2 increase
LY294002 increase
Downstream Regulation
Effect of modification (process):  transcription, inhibited

K408-ac - FOXO4 (mouse)
Modsite: GGMPSSSkLGTGVSL SwissProt Entrez-Gene
Orthologous residues
FOXO4 (human): K407‑ac, FOXO4 (mouse): K408‑ac, FOXO4 (rat): K408‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial), HeLa (cervical), Jurkat (T lymphocyte), Saos-2 (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
DEACETYLASE SIRT1 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
ACETYLTRANSFERASE p300 (mouse) transfection of wild-type enzyme
DEACETYLASE SIRT1 (mouse) pharmacological activator of upstream enzyme, transfection of inactive enzyme, pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme, co-immunoprecipitation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nicotinamide increase
resveratrol decrease
H2O2 increase
LY294002 increase
Downstream Regulation
Effect of modification (process):  transcription, inhibited