Curated Information
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Home > Curated Information Page > PubMed Id: 19111882
Otto T, et al. (2009) Stabilization of N-Myc is a critical function of Aurora A in human neuroblastoma. Cancer Cell 15, 67-78 19111882
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T395-p - CCNE1 (human)
Modsite: PLPSGLLtPPQsGKK SwissProt Entrez-Gene
Orthologous residues
CCNE1 (human): T395‑p, CCNE1 (mouse): T393‑p, CCNE1 (rat): T396‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  SHEP (neuron)
Cellular systems studied:  cell lines
Species studied:  human

T58-p - N-Myc (human)
Modsite: KkFELLPtPPLsPsr SwissProt Entrez-Gene
Orthologous residues
N‑Myc (human): T58‑p, N‑Myc (mouse): T58‑p, N‑Myc (rat): T58‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  IMR32 (neural crest), SHEP (neuron)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MG132 increase
MG132 N-Myc (human) increase
nocodazole MG132 no effect upon treatment-induced increase
nocodazole N-Myc (human) no effect upon treatment-induced increase
siRNA decrease Aurora A siRNA
LY294002 decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation, ubiquitination
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
AurA (human) Induces molecular association, regulation, protein stabilization co-immunoprecipitation

S62-p - N-Myc (human)
Modsite: LLPtPPLsPsrGFAE SwissProt Entrez-Gene
Orthologous residues
N‑Myc (human): S62‑p, N‑Myc (mouse): S62‑p, N‑Myc (rat): S62‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  IMR32 (neural crest), SHEP (neuron)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
siRNA decrease Aurora A siRNA
LY294002 decrease
nocodazole increase
MG132 nocodazole no effect upon treatment-induced increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation, ubiquitination
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
AurA (human) Induces molecular association, regulation, protein stabilization co-immunoprecipitation