Liu Q, Bee MS, Schonbrunn A (2009) Site specificity of agonist and second messenger-activated kinases for somatostatin receptor subtype 2A (Sst2A) phosphorylation. Mol Pharmacol 76, 68-80 19389921

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S341-p - SSTR2 (rat) ▲

Modsite: GAEDGERsDsKQDKs SwissProt Orthologous residues SSTR2 (human): S341‑p, SSTR2 (mouse): S341‑p, SSTR2 (rat): S341‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), CHO (fibroblast), GH4C1 (pituitary cell) [SSTR2 (rat), transfection] Cellular systems studied:  cell lines Species studied:  hamster Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GRK2 (human) siRNA inhibition of enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes SST14 increase

S343-p - SSTR2 (rat) ▲

Modsite: EDGERsDsKQDKsRL SwissProt Orthologous residues SSTR2 (human): S343‑p, SSTR2 (mouse): S343‑p, SSTR2 (rat): S343‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), CHO (fibroblast), GH4C1 (pituitary cell) [SSTR2 (rat), transfection] Cellular systems studied:  cell lines Species studied:  hamster Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GRK2 (human) siRNA inhibition of enzyme KINASE PKCA (human) pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase GF109203X phorbol_ester inhibit treatment-induced increase PTX phorbol_ester no effect upon treatment-induced increase SST14 increase GF109203X SST14 no effect upon treatment-induced increase PTX SST14 no effect upon treatment-induced increase

S348-p - SSTR2 (rat) ▲

Modsite: sDsKQDKsRLNEttE SwissProt Orthologous residues SSTR2 (human): S348‑p, SSTR2 (mouse): S348‑p, SSTR2 (rat): S348‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), CHO (fibroblast), GH4C1 (pituitary cell) [SSTR2 (rat), transfection] Cellular systems studied:  cell lines Species studied:  hamster Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes SST14 increase phorbol_ester increase

T353-p - SSTR2 (rat) ▲

Modsite: DKsRLNEttEtQRtL SwissProt Orthologous residues SSTR2 (human): T353‑p, SSTR2 (mouse): T353‑p, SSTR2 (rat): T353‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), CHO (fibroblast), GH4C1 (pituitary cell) [SSTR2 (rat), transfection] Cellular systems studied:  cell lines Species studied:  hamster Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes SST14 increase

T354-p - SSTR2 (rat) ▲

Modsite: KsRLNEttEtQRtLL SwissProt Orthologous residues SSTR2 (human): T354‑p, SSTR2 (mouse): T354‑p, SSTR2 (rat): T354‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), CHO (fibroblast), GH4C1 (pituitary cell) [SSTR2 (rat), transfection] Cellular systems studied:  cell lines Species studied:  hamster Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes SST14 increase