Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 19389921
Liu Q, Bee MS, Schonbrunn A (2009) Site specificity of agonist and second messenger-activated kinases for somatostatin receptor subtype 2A (Sst2A) phosphorylation. Mol Pharmacol 76, 68-80 19389921
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S341-p - SSTR2 (rat)
Modsite: GAEDGERsDsKQDKs SwissProt
Orthologous residues
SSTR2 (human): S341‑p, SSTR2 (mouse): S341‑p, SSTR2 (rat): S341‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), CHO (fibroblast), GH4C1 (pituitary cell) [SSTR2 (rat), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GRK2 (human) siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SST14 increase

S343-p - SSTR2 (rat)
Modsite: EDGERsDsKQDKsRL SwissProt
Orthologous residues
SSTR2 (human): S343‑p, SSTR2 (mouse): S343‑p, SSTR2 (rat): S343‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), CHO (fibroblast), GH4C1 (pituitary cell) [SSTR2 (rat), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GRK2 (human) siRNA inhibition of enzyme
KINASE PKCA (human) pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
GF109203X phorbol_ester inhibit treatment-induced increase
PTX phorbol_ester no effect upon treatment-induced increase
SST14 increase
GF109203X SST14 no effect upon treatment-induced increase
PTX SST14 no effect upon treatment-induced increase

S348-p - SSTR2 (rat)
Modsite: sDsKQDKsRLNEttE SwissProt
Orthologous residues
SSTR2 (human): S348‑p, SSTR2 (mouse): S348‑p, SSTR2 (rat): S348‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), CHO (fibroblast), GH4C1 (pituitary cell) [SSTR2 (rat), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SST14 increase
phorbol_ester increase

T353-p - SSTR2 (rat)
Modsite: DKsRLNEttEtQRtL SwissProt
Orthologous residues
SSTR2 (human): T353‑p, SSTR2 (mouse): T353‑p, SSTR2 (rat): T353‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), CHO (fibroblast), GH4C1 (pituitary cell) [SSTR2 (rat), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SST14 increase

T354-p - SSTR2 (rat)
Modsite: KsRLNEttEtQRtLL SwissProt
Orthologous residues
SSTR2 (human): T354‑p, SSTR2 (mouse): T354‑p, SSTR2 (rat): T354‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), CHO (fibroblast), GH4C1 (pituitary cell) [SSTR2 (rat), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SST14 increase