Curated Information
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Home > Curated Information Page > PubMed Id: 17287208
Fang D, et al. (2007) Phosphorylation of beta-catenin by AKT promotes beta-catenin transcriptional activity. J Biol Chem 282, 11221-9 17287208
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S552-p - CTNNB1 (human)
Modsite: QDtQRRtsMGGtQQQ SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): S552‑p, CTNNB1 (mouse): S552‑p, CTNNB1 (rat): S552‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293T (epithelial), A431 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
KINASE Akt2 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) mutation in upstream enzyme recognition motif, transfection of constitutively active enzyme, pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme
KINASE Akt2 (human) mutation in upstream enzyme recognition motif, transfection of constitutively active enzyme, pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  cell motility, altered, transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 zeta (human) Induces co-immunoprecipitation
Comments:  S552-P translocates from cell contacts to the cytosol and nucleus. Constitutively active Akt enhanced tumor cell invasion.

S675-p - CTNNB1 (human)
Modsite: QDykKRLsVELtsSL SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): S675‑p, CTNNB1 (mouse): S675‑p, CTNNB1 (rat): S675‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293T (epithelial), A431 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (human)