Lu M, Shyy JY (2006) Sterol regulatory element-binding protein 1 is negatively modulated by PKA phosphorylation. Am J Physiol Cell Physiol 290, C1477-86 16381800

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S338-p - SREBP-1 (human) ▲

Modsite: IEkRYRSsINDkIIE SwissProt Entrez-Gene Orthologous residues SREBP‑1 (human): S338‑p, SREBP‑1 iso3 (human): S314‑p, SREBP‑1 iso4 (human): S368‑p, SREBP‑1 (mouse): S332‑p, SREBP‑1 iso4 (mouse): S266‑p, SREBP‑1 (rat): S332‑p, SREBP‑1 iso1 (rat): S308‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody Relevant cell lines - cell types - tissues:  293 (epithelial), HepG2 (hepatic) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKACA (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKACA (human) pharmacological activator of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes okadaic_acid, colforsin increase Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, inhibited Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays SREBP-1 (human) Disrupts transcription, altered pull-down assay Comments:  inhibits lipogenesis

S314-p - SREBP-1 iso3 (human) ▲

Modsite: IEKRYRSsINDKIIE SwissProt Entrez-Gene Orthologous residues SREBP‑1 (human): S338‑p, SREBP‑1 iso3 (human): S314‑p, SREBP‑1 iso4 (human): S368‑p, SREBP‑1 (mouse): S332‑p, SREBP‑1 iso4 (mouse): S266‑p, SREBP‑1 (rat): S332‑p, SREBP‑1 iso1 (rat): S308‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody Relevant cell lines - cell types - tissues:  293 (epithelial), HepG2 (hepatic) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKACA (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKACA (human) pharmacological activator of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes okadaic_acid, colforsin increase Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, inhibited Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays SREBP-1 iso3 (human) Disrupts transcription, altered pull-down assay Comments:  inhibits lipogenesis