Curated Information
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Home > Curated Information Page > PubMed Id: 16357133
Palamarchuk A, et al. (2005) Akt phosphorylates and regulates Pdcd4 tumor suppressor protein. Cancer Res 65, 11282-6 16357133
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S67-p - PDCD4 (human)
Modsite: kRRLRKNssRDsGrG SwissProt Entrez-Gene
Orthologous residues
PDCD4 (human): S67‑p, PDCD4 iso2 (human): S56‑p, PDCD4 (mouse): S67‑p, PDCD4 (rat): S67‑p, PDCD4 (cow): S67‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) phospho-motif antibody
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin insulin inhibit treatment-induced increase

S457-p - PDCD4 (human)
Modsite: RGRKRFVsEGDGGRL SwissProt Entrez-Gene
Orthologous residues
PDCD4 (human): S457‑p, PDCD4 iso2 (human): S446‑p, PDCD4 (mouse): S457‑p, PDCD4 (rat): S457‑p, PDCD4 (cow): S457‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) phospho-motif antibody
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin insulin inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  inhibits repressor activity of PDCD4 on AP-1 responsive promoters