Curated Information
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Home > Curated Information Page > PubMed Id: 10319993
Blake RA, Garcia-Paramio P, Parker PJ, Courtneidge SA (1999) Src promotes PKCdelta degradation. Cell Growth Differ 10, 231-41 10319993
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y311-p - PKCD (rat)
Modsite: TPETVGIyQGFEKKT SwissProt Entrez-Gene
Orthologous residues
PKCD (human): Y313‑p, PKCD iso2 (human): Y313‑p, PKCD (mouse): Y311‑p, PKCD iso2 (mouse): Y311‑p, PKCD (rat): Y311‑p
Characterization
Methods used to characterize site in vivo flow cytometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) mutation in upstream enzyme recognition motif, transfection of constitutively active enzyme, pharmacological activator of upstream enzyme
Comments:  Processive phosphorylation of PKCD residues starting with Y311.
Downstream Regulation
Effect of modification (function):  protein degradation

Y372-p - PKCD (rat)
Modsite: ELKGKERyFAIKYLK SwissProt Entrez-Gene
Orthologous residues
PKCD (human): Y374‑p, PKCD iso2 (human): Y405‑p, PKCD (mouse): Y372‑p, PKCD iso2 (mouse): Y398‑p, PKCD (rat): Y372‑p
Characterization
Methods used to characterize site in vivo flow cytometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) mutation in upstream enzyme recognition motif, transfection of constitutively active enzyme, pharmacological activator of upstream enzyme
Comments:  Processive phosphorylation of PKCD residues starting with Y311.

Y565-p - PKCD (rat)
Modsite: IRVDTPHyPRWITKE SwissProt Entrez-Gene
Orthologous residues
PKCD (human): Y567‑p, PKCD iso2 (human): Y598‑p, PKCD (mouse): Y565‑p, PKCD iso2 (mouse): Y591‑p, PKCD (rat): Y565‑p
Characterization
Methods used to characterize site in vivo flow cytometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) mutation in upstream enzyme recognition motif, transfection of constitutively active enzyme, pharmacological activator of upstream enzyme
Comments:  Processive phosphorylation of PKCD residues starting with Y311.

Y628-p - PKCD (rat)
Modsite: KVKSPSDySNFDPEF SwissProt Entrez-Gene
Orthologous residues
PKCD (human): Y630‑p, PKCD iso2 (human): Y661‑p, PKCD (mouse): Y628‑p, PKCD iso2 (mouse): Y654‑p, PKCD (rat): Y628‑p
Characterization
Methods used to characterize site in vivo flow cytometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) mutation in upstream enzyme recognition motif, transfection of constitutively active enzyme, pharmacological activator of upstream enzyme
Comments:  Processive phosphorylation of PKCD residues starting with Y311.