Curated Information
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Home > Curated Information Page > PubMed Id: 10508618
Jones N, Dumont DJ (1999) Recruitment of Dok-R to the EGF receptor through its PTB domain is required for attenuation of Erk MAP kinase activation. Curr Biol 9, 1057-60 10508618
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y1110-p - EGFR (human)
Modsite: GsVQNPVyHNQPLNP SwissProt Entrez-Gene
Orthologous residues
EGFR (human): Y1110‑p, EGFR iso2 (human): , EGFR iso5 (human): , EGFR (mouse): Y1110‑p, EGFR (rat): Y1109‑p, EGFR (pig): Y1109‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Comments:  in vitro and in vivo binding studies
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DOK2 (mouse) PTB Induces in vitro, pull-down assay

Y1172-p - EGFR (human)
Modsite: IsLDNPDyQQDFFPk SwissProt Entrez-Gene
Orthologous residues
EGFR (human): Y1172‑p, EGFR iso2 (human): , EGFR iso5 (human): , EGFR (mouse): Y1172‑p, EGFR (rat): Y1171‑p, EGFR (pig): Y1171‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Comments:  in vitro and in vivo binding studies
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DOK2 (mouse) PTB Induces in vitro, pull-down assay

Y1197-p - EGFR (human)
Modsite: stAENAEyLRVAPQS SwissProt Entrez-Gene
Orthologous residues
EGFR (human): Y1197‑p, EGFR iso2 (human): , EGFR iso5 (human): , EGFR (mouse): Y1197‑p, EGFR (rat): Y1196‑p, EGFR (pig): Y1196‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Comments:  in vitro and in vivo binding studies

T202-p - ERK1 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase WT and DM mutant (cannot bind rasGAP)

Y204-p - ERK1 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase WT and DM mutant (cannot bind rasGAP)

T185-p - ERK2 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase WT and DM mutant (cannot bind rasGAP)

Y187-p - ERK2 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase WT and DM mutant (cannot bind rasGAP)

Y276-p - DOK2 (mouse)
Modsite: LPRPESPySRPHDSL SwissProt Entrez-Gene
Orthologous residues
DOK2 (human): Y271‑p, DOK2 (mouse): Y276‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Comments:  in vitro and in vivo binding studies
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
RASA1 (human) SH2 Induces molecular association, regulation in vitro, pull-down assay
Comments:  after EGF stimulation

Y304-p - DOK2 (mouse)
Modsite: PGAPEGEyAVPFDTV SwissProt Entrez-Gene
Orthologous residues
DOK2 (human): Y299‑p, DOK2 (mouse): Y304‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Comments:  in vitro and in vivo binding studies
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
RASA1 (human) SH2 Induces molecular association, regulation pull-down assay, in vitro
Comments:  after EGF stimulation

Y351-p - DOK2 (mouse)
Modsite: APLPDHIyDEPEGVA SwissProt Entrez-Gene
Orthologous residues
DOK2 (human): Y345‑p, DOK2 (mouse): Y351‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Comments:  in vitro and in vivo binding studies
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
NCK1 (human) SH2 Induces molecular association, regulation co-immunoprecipitation, in vitro
Comments:  association in vitro and in vivo after EGF stimulation