Curated Information
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Home > Curated Information Page > PubMed Id: 19285946
Woodcock SA, et al. (2009) SRC-induced disassembly of adherens junctions requires localized phosphorylation and degradation of the rac activator tiam1. Mol Cell 33, 639-53 19285946
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y384-p - TIAM1 (mouse)
Modsite: DRARQGVyENFRREL SwissProt Entrez-Gene
Orthologous residues
TIAM1 (human): Y384‑p, TIAM1 (mouse): Y384‑p, TIAM1 (rat): Y383‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293T (epithelial), MDCK (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Src (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
vanadate increase
SU6656 vanadate inhibit treatment-induced increase
PP2 vanadate inhibit treatment-induced increase
HGF increase
SU6656 HGF inhibit treatment-induced increase
PP2 HGF inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation, protein degradation
Effect of modification (process):  cell motility, altered, cytoskeletal reorganization
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
GRB2 (human) Induces co-immunoprecipitation
ERK1 (human) Induces enzymatic activity, induced co-immunoprecipitation
ERK2 (human) Induces enzymatic activity, induced co-immunoprecipitation
Comments:  Grb2-Sos1