Wong RH, et al. (2009) A role of DNA-PK for the metabolic gene regulation in response to insulin. Cell 136, 1056-72 19303849

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K235-ac - USF1 (mouse) ▲

Modsite: DCSMESTkSGQSKGG SwissProt Entrez-Gene Orthologous residues USF1 (human): K235‑ac, USF1 (mouse): K235‑ac, USF1 (rat): K235‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site Disease tissue studied:  brain cancer, glioblastoma, glioma, liver cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HepG2 (hepatic), M059J (glial), M059K (glial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE PCAF (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE PCAF (human) transfection of wild-type enzyme, co-immunoprecipitation DEACETYLASE HDAC9 (human) transfection of wild-type enzyme, co-immunoprecipitation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes meal feeding increase insulin increase Downstream Regulation Effect of modification (process):  transcription, altered

S262-p - USF1 (mouse) ▲

Modsite: RQSNHRLsEELQGLD SwissProt Entrez-Gene Orthologous residues USF1 (human): S262‑p, USF1 (mouse): S262‑p, USF1 (rat): S262‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site, western blotting Disease tissue studied:  brain cancer, glioblastoma, glioma, liver cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HepG2 (hepatic), liver, M059J (glial), M059K (glial) Cellular systems studied:  cell lines, tissue Species studied:  human, mouse Comments:  only in fed mice Enzymes shown to modify site in vitro:  Type Enzyme KINASE DNAPK (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE DNAPK (human) siRNA inhibition of enzyme, transfection of constitutively active enzyme, transfection of dominant-negative enzyme, activation of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes meal feeding increase siRNA meal feeding inhibit treatment-induced increase DNA-PK siRNA okadaic_acid decrease tautomycin decrease insulin increase Downstream Regulation Effect of modification (function):  acetylation, molecular association, regulation Effect of modification (process):  transcription, altered Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays SREBP-1 (human) Induces co-immunoprecipitation