Curated Information
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Home > Curated Information Page > PubMed Id: 15001356
Burch LR, et al. (2004) Phage-peptide display identifies the interferon-responsive, death-activated protein kinase family as a novel modifier of MDM2 and p21WAF1. J Mol Biol 337, 115-28 15001356
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S166-p - MDM2 (human)
Modsite: SsRRRAIsEtEENsD SwissProt Entrez-Gene
Orthologous residues
MDM2 (human): S166‑p, MDM2 (mouse): S163‑p, MDM2 (rat): S138‑p

S186-p - MDM2 (human)
Modsite: RQRkRHksDsIsLsF SwissProt Entrez-Gene
Orthologous residues
MDM2 (human): S186‑p, MDM2 (mouse): S183‑p, MDM2 (rat): A158‑p

T145-p - p21Cip1 (human)
Modsite: QGRkRRQtsMTDFyH SwissProt Entrez-Gene
Orthologous residues
p21Cip1 (human): T145‑p, p21Cip1 (mouse): T140‑p, p21Cip1 (dog): T113‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE DAPK3 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE DAPK3 (human) transfection of wild-type enzyme, phospho-antibody, modification site within consensus motif
Downstream Regulation
Effect of modification (function):  protein stabilization