Curated Information
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Home > Curated Information Page > PubMed Id: 19339993
Wu SY, Chiang CM (2009) Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding. EMBO J 28, 1246-59 19339993
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K120-ac - p53 (human)
Modsite: FLhSGTAkSVTCTyS SwissProt Entrez-Gene
Orthologous residues
p53 (human): K120‑ac, p53 iso2 (human): K120‑ac, p53 iso4 (human): K81‑ac, p53 (mouse): K117‑ac, p53 iso2 (mouse): K117‑ac, p53 (rat): K118‑ac, p53 (rabbit): K117‑ac, p53 (green monkey): K120‑ac
Characterization
Methods used to characterize site in vivo mutation of modification site
Cellular systems studied:  cell lines
Species studied:  insect
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE Tip60 (human)
Downstream Regulation
Effect of modification (process):  transcription, induced

K164-ac - p53 (human)
Modsite: VRAMAIYkQSQHMTE SwissProt Entrez-Gene
Orthologous residues
p53 (human): K164‑ac, p53 iso2 (human): K164‑ac, p53 iso4 (human): K125‑ac, p53 (mouse): K161‑ac, p53 iso2 (mouse): K161‑ac, p53 (rat): K162‑ac, p53 (rabbit): K161‑ac, p53 (green monkey): K164‑ac
Characterization
Methods used to characterize site in vivo mutation of modification site
Cellular systems studied:  cell lines
Species studied:  insect
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (human)
Downstream Regulation
Effect of modification (process):  transcription, induced

K305-ac - p53 (human)
Modsite: ELPPGStkRALPNNt SwissProt Entrez-Gene
Orthologous residues
p53 (human): K305‑ac, p53 iso2 (human): K305‑ac, p53 iso4 (human): K266‑ac, p53 (mouse): K302‑ac, p53 iso2 (mouse): K302‑ac, p53 (rat): K303‑ac, p53 (rabbit): K302‑ac, p53 (green monkey): K305‑ac
Characterization
Methods used to characterize site in vivo mutation of modification site
Cellular systems studied:  cell lines
Species studied:  insect
Downstream Regulation
Effect of modification (process):  transcription, induced

K320-ac - p53 (human)
Modsite: sssPQPkkkPLDGEy SwissProt Entrez-Gene
Orthologous residues
p53 (human): K320‑ac, p53 iso2 (human): K320‑ac, p53 iso4 (human): K281‑ac, p53 (mouse): K317‑ac, p53 iso2 (mouse): K317‑ac, p53 (rat): K318‑ac, p53 (rabbit): K318‑ac, p53 (green monkey): K320‑ac
Characterization
Methods used to characterize site in vivo mutation of modification site
Cellular systems studied:  cell lines
Species studied:  insect
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE PCAF (human)
Downstream Regulation
Effect of modification (process):  transcription, induced

K370-ac - p53 (human)
Modsite: RAHsSHLkskkGQst SwissProt Entrez-Gene
Orthologous residues
p53 (human): K370‑ac, p53 iso2 (human): , p53 iso4 (human): K331‑ac, p53 (mouse): K367‑ac, p53 iso2 (mouse): , p53 (rat): K368‑ac, p53 (rabbit): K368‑ac, p53 (green monkey): K370‑ac
Characterization
Methods used to characterize site in vivo mutation of modification site
Cellular systems studied:  cell lines
Species studied:  insect
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (human)
Downstream Regulation
Effect of modification (process):  transcription, induced

K372-ac - p53 (human)
Modsite: HsSHLkskkGQstsR SwissProt Entrez-Gene
Orthologous residues
p53 (human): K372‑ac, p53 iso2 (human): , p53 iso4 (human): K333‑ac, p53 (mouse): K369‑ac, p53 iso2 (mouse): , p53 (rat): K370‑ac, p53 (rabbit): K370‑ac, p53 (green monkey): K372‑ac
Characterization
Methods used to characterize site in vivo mutation of modification site
Cellular systems studied:  cell lines
Species studied:  insect
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (human)
Downstream Regulation
Effect of modification (process):  transcription, induced

K373-ac - p53 (human)
Modsite: sSHLkskkGQstsRH SwissProt Entrez-Gene
Orthologous residues
p53 (human): K373‑ac, p53 iso2 (human): , p53 iso4 (human): K334‑ac, p53 (mouse): K370‑ac, p53 iso2 (mouse): , p53 (rat): K371‑ac, p53 (rabbit): K371‑ac, p53 (green monkey): K373‑ac
Characterization
Methods used to characterize site in vivo mutation of modification site
Cellular systems studied:  cell lines
Species studied:  insect
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (human)
Downstream Regulation
Effect of modification (process):  transcription, induced

K381-ac - p53 (human)
Modsite: GQstsRHkkLMFktE SwissProt Entrez-Gene
Orthologous residues
p53 (human): K381‑ac, p53 iso2 (human): , p53 iso4 (human): K342‑ac, p53 (mouse): K378‑ac, p53 iso2 (mouse): , p53 (rat): K379‑ac, p53 (rabbit): K379‑ac, p53 (green monkey): K381‑ac
Characterization
Methods used to characterize site in vivo mutation of modification site
Cellular systems studied:  cell lines
Species studied:  insect
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (human)
Downstream Regulation
Effect of modification (process):  transcription, induced

K382-ac - p53 (human)
Modsite: QstsRHkkLMFktEG SwissProt Entrez-Gene
Orthologous residues
p53 (human): K382‑ac, p53 iso2 (human): , p53 iso4 (human): K343‑ac, p53 (mouse): K379‑ac, p53 iso2 (mouse): , p53 (rat): K380‑ac, p53 (rabbit): K380‑ac, p53 (green monkey): K382‑ac
Characterization
Methods used to characterize site in vivo mutation of modification site
Cellular systems studied:  cell lines
Species studied:  insect
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (human)
Downstream Regulation
Effect of modification (process):  transcription, induced

K386-ac - p53 (human)
Modsite: RHkkLMFktEGPDsD SwissProt Entrez-Gene
Orthologous residues
p53 (human): K386‑ac, p53 iso2 (human): , p53 iso4 (human): K347‑ac, p53 (mouse): K383‑ac, p53 iso2 (mouse): , p53 (rat): K384‑ac, p53 (rabbit): K384‑ac, p53 (green monkey): K386‑ac
Characterization
Methods used to characterize site in vivo mutation of modification site
Cellular systems studied:  cell lines
Species studied:  insect
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (human)
Downstream Regulation
Effect of modification (process):  transcription, induced

K386-sm - p53 (human)
Modsite: RHkkLMFktEGPDsD SwissProt Entrez-Gene
Orthologous residues
p53 (human): K386‑sm, p53 iso2 (human): , p53 iso4 (human): K347‑sm, p53 (mouse): K383‑sm, p53 iso2 (mouse): , p53 (rat): K384‑sm, p53 (rabbit): K384‑sm, p53 (green monkey): K386‑sm
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Disease tissue studied:  bone cancer, colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal), Saos-2 (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
DESUMOYLASE SENP1 (human)
SUMO LIGASE UBC9 (human)
SUMO LIGASE UBLE1A (human)
SUMO LIGASE SAE2 (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts transcription, inhibited EMSA