Curated Information
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Home > Curated Information Page > PubMed Id: 19118035
Kim J, et al. (2009) Cytoplasmic sequestration of p27 via AKT phosphorylation in renal cell carcinoma. Clin Cancer Res 15, 81-90 19118035
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  kidney cancer
Relevant cell lines - cell types - tissues:  Caki-1 (renal), kidney, RCC4 (renal)
Cellular systems studied:  cell lines, tissue
Species studied:  human
Comments:  normal and tumor kidney
Associated Diseases
Diseases Alterations Comments
kidney cancer increased renal cell carcinoma

T157-p - p27Kip1 (human)
Modsite: GIRkrPAtDDSSTQN SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T157‑p, p27Kip1 (mouse): A157‑p, p27Kip1 (rat): A157‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LY294002 decrease
wortmannin decrease
rapamycin decrease
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  apoptosis, inhibited
Comments:  cytoplasmic localization
Associated Diseases
Diseases Alterations Comments
kidney cancer increased renal cell carcinoma

T198-p - p27Kip1 (human)
Modsite: PGLRRRQt_______ SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T198‑p, p27Kip1 (mouse): T197‑p, p27Kip1 (rat): T197‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  kidney cancer
Relevant cell lines - cell types - tissues:  Caki-1 (renal), kidney, RCC4 (renal)
Cellular systems studied:  cell lines, tissue
Species studied:  human
Comments:  normal and tumor kidney
Associated Diseases
Diseases Alterations Comments
kidney cancer decreased renal cell carcinoma

S235-p - S6 (human)
Modsite: IAKRRRLssLRAsts SwissProt Entrez-Gene
Orthologous residues
S6 (human): S235‑p, S6 (mouse): S235‑p, S6 (rat): S235‑p, S6 (fruit fly): A234‑p, S6 (cow): S235‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  kidney cancer
Relevant cell lines - cell types - tissues:  Caki-1 (renal), kidney, RCC4 (renal)
Cellular systems studied:  cell lines, tissue
Species studied:  human
Comments:  normal and tumor kidney

S236-p - S6 (human)
Modsite: AKRRRLssLRAstsK SwissProt Entrez-Gene
Orthologous residues
S6 (human): S236‑p, S6 (mouse): S236‑p, S6 (rat): S236‑p, S6 (fruit fly): S235‑p, S6 (cow): S236‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  kidney cancer
Relevant cell lines - cell types - tissues:  Caki-1 (renal), kidney, RCC4 (renal)
Cellular systems studied:  cell lines, tissue
Species studied:  human
Comments:  normal and tumor kidney