Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.2
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 19124463
Ros S, et al. (2009) Control of liver glycogen synthase activity and intracellular distribution by phosphorylation. J Biol Chem 284, 6370-8 19124463
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S8-p - GYS2 (rat)
Modsite: MLRGRsLsVtsLGGL SwissProt Entrez-Gene
Orthologous residues
GYS2 (human): S8‑p, GYS2 (mouse): S8‑p, GYS2 (rat): S8‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  hepatocyte-liver
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
glucose_starvation increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, inhibited, intracellular localization
Comments:  translocation dependent on glycogen synthesis

S11-p - GYS2 (rat)
Modsite: GRsLsVtsLGGLPAW SwissProt Entrez-Gene
Orthologous residues
GYS2 (human): S11‑p, GYS2 (mouse): S11‑p, GYS2 (rat): S11‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  hepatocyte-liver
Cellular systems studied:  primary cultured cells
Species studied:  rat

S641-p - GYS2 (rat)
Modsite: FKYPRPssVPPsPSG SwissProt Entrez-Gene
Orthologous residues
GYS2 (human): S641‑p, GYS2 (mouse): S641‑p, GYS2 (rat): S641‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  hepatocyte-liver
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
glucose_starvation increase
Downstream Regulation
Effect of modification (function):  phosphorylation
Comments:  S641A mutant caused decrease in S645 phosphorylation

S645-p - GYS2 (rat)
Modsite: RPssVPPsPSGSQtS SwissProt Entrez-Gene
Orthologous residues
GYS2 (human): S645‑p, GYS2 (mouse): S645‑p, GYS2 (rat): S645‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  hepatocyte-liver
Cellular systems studied:  primary cultured cells
Species studied:  rat