Abdelmohsen K, et al. (2005) Doxorubicin induces EGF receptor-dependent downregulation of gap junctional intercellular communication in rat liver epithelial cells. Biol Chem 386, 217-23 15843167

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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T203-p - ERK1 (rat) ▲

Modsite: HDHTGFLtEyVAtRW SwissProt Entrez-Gene Orthologous residues ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  WB-F344 (epithelial) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes adriamycin increase U0126 adriamycin inhibit treatment-induced increase AG1478 adriamycin inhibit treatment-induced increase compound_56 adriamycin inhibit treatment-induced increase

Y205-p - ERK1 (rat) ▲

Modsite: HTGFLtEyVAtRWYR SwissProt Entrez-Gene Orthologous residues ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  WB-F344 (epithelial) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes adriamycin increase U0126 adriamycin inhibit treatment-induced increase AG1478 adriamycin inhibit treatment-induced increase compound_56 adriamycin inhibit treatment-induced increase

T183-p - ERK2 (rat) ▲

Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene Orthologous residues ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  WB-F344 (epithelial) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes adriamycin increase U0126 adriamycin inhibit treatment-induced increase AG1478 adriamycin inhibit treatment-induced increase compound_56 adriamycin inhibit treatment-induced increase

Y185-p - ERK2 (rat) ▲

Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene Orthologous residues ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  WB-F344 (epithelial) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes adriamycin increase U0126 adriamycin inhibit treatment-induced increase AG1478 adriamycin inhibit treatment-induced increase compound_56 adriamycin inhibit treatment-induced increase

S279-p - GJA1 (rat) ▲

Modsite: SSPTAPLsPMsPPGY SwissProt Entrez-Gene Orthologous residues GJA1 (human): S279‑p, GJA1 (mouse): S279‑p, GJA1 (rat): S279‑p, GJA1 (rabbit): S279‑p, GJA1 (pig): S279‑p, GJA1 (hamster): S279‑p, GJA1 (cow): S280‑p Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  WB-F344 (epithelial) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE ERK2 (rat) pharmacological activator of upstream enzyme KINASE ERK1 (rat) pharmacological activator of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes adriamycin no change compared to control menadione increase

S282-p - GJA1 (rat) ▲

Modsite: TAPLsPMsPPGYkLV SwissProt Entrez-Gene Orthologous residues GJA1 (human): S282‑p, GJA1 (mouse): S282‑p, GJA1 (rat): S282‑p, GJA1 (rabbit): S282‑p, GJA1 (pig): S282‑p, GJA1 (hamster): S282‑p, GJA1 (cow): S283‑p Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  WB-F344 (epithelial) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE ERK2 (rat) pharmacological activator of upstream enzyme KINASE ERK1 (rat) pharmacological activator of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes adriamycin no change compared to control menadione increase