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Home > Curated Information Page > PubMed Id: 19186949
Platt MD, Salicioni AM, Hunt DF, Visconti PE (2009) Use of differential isotopic labeling and mass spectrometry to analyze capacitation-associated changes in the phosphorylation status of mouse sperm proteins. J Proteome Res 8, 1431-40 19186949
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S65-p - AKAP4 (mouse)
Modsite: KGAAGSRsEGELNLE SwissProt Entrez-Gene
Orthologous residues
AKAP4 (human): S65‑p, AKAP4 (mouse): S65‑p, AKAP4 (rat): S64‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S226-p - AKAP4 (mouse)
Modsite: FYVNRLSsLVIQMAR SwissProt Entrez-Gene
Orthologous residues
AKAP4 (human): S226‑p, AKAP4 (mouse): S226‑p, AKAP4 (rat): S225‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S812-p - AKAP4 (mouse)
Modsite: KAAEKGYsVGDLLQE SwissProt Entrez-Gene
Orthologous residues
AKAP4 (human): S817‑p, AKAP4 (mouse): S812‑p, AKAP4 (rat): S810‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

T238-p - CABYR (mouse)
Modsite: EVQVVSKtsAQVVVG SwissProt Entrez-Gene
Orthologous residues
CABYR (human): T239‑p, CABYR iso2 (human): T221‑p, CABYR iso3 (human): , CABYR (mouse): T238‑p, CABYR iso2 (mouse): , CABYR (rat): T237‑p

S239-p - CABYR (mouse)
Modsite: VQVVSKtsAQVVVGP SwissProt Entrez-Gene
Orthologous residues
CABYR (human): S240‑p, CABYR iso2 (human): S222‑p, CABYR iso3 (human): , CABYR (mouse): S239‑p, CABYR iso2 (mouse): , CABYR (rat): S238‑p

S324-p - CABYR (mouse)
Modsite: CVKEIEQsPPGsPKA SwissProt Entrez-Gene
Orthologous residues
CABYR (human): S339‑p, CABYR iso2 (human): S321‑p, CABYR iso3 (human): , CABYR (mouse): S324‑p, CABYR iso2 (mouse): , CABYR (rat): S322‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S328-p - CABYR (mouse)
Modsite: IEQsPPGsPKAVEPK SwissProt Entrez-Gene
Orthologous residues
CABYR (human): S343‑p, CABYR iso2 (human): S325‑p, CABYR iso3 (human): , CABYR (mouse): S328‑p, CABYR iso2 (mouse): , CABYR (rat): S326‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S339-p - CABYR (mouse)
Modsite: VEPKTKIsIEsLKTV SwissProt Entrez-Gene
Orthologous residues
CABYR (human): S359‑p, CABYR iso2 (human): S341‑p, CABYR iso3 (human): , CABYR (mouse): S339‑p, CABYR iso2 (mouse): , CABYR (rat): S337‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S342-p - CABYR (mouse)
Modsite: KTKIsIEsLKTVQVE SwissProt Entrez-Gene
Orthologous residues
CABYR (human): S362‑p, CABYR iso2 (human): S344‑p, CABYR iso3 (human): , CABYR (mouse): S342‑p, CABYR iso2 (mouse): , CABYR (rat): S340‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S757-p - CCDC136 (mouse)
Modsite: STENFHRsyEssIDE SwissProt Entrez-Gene
Orthologous residues
CCDC136 (human): T761‑p, CCDC136 iso4 (human): T761‑p, CCDC136 (mouse): S757‑p, CCDC136 (rat): S714‑p

Y758-p - CCDC136 (mouse)
Modsite: TENFHRsyEssIDEN SwissProt Entrez-Gene
Orthologous residues
CCDC136 (human): Y762‑p, CCDC136 iso4 (human): Y762‑p, CCDC136 (mouse): Y758‑p, CCDC136 (rat): Y715‑p

S760-p - CCDC136 (mouse)
Modsite: NFHRsyEssIDENEG SwissProt Entrez-Gene
Orthologous residues
CCDC136 (human): T764‑p, CCDC136 iso4 (human): T764‑p, CCDC136 (mouse): S760‑p, CCDC136 (rat): S717‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S761-p - CCDC136 (mouse)
Modsite: FHRsyEssIDENEGY SwissProt Entrez-Gene
Orthologous residues
CCDC136 (human): T765‑p, CCDC136 iso4 (human): T765‑p, CCDC136 (mouse): S761‑p, CCDC136 (rat): T718‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S101-p - CCDC27 (mouse)
Modsite: QFSTRTTsMSHVGsA SwissProt Entrez-Gene
Orthologous residues
CCDC27 (human): S143‑p, CCDC27 (mouse): S101‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S107-p - CCDC27 (mouse)
Modsite: TsMSHVGsAIMVDLP SwissProt Entrez-Gene
Orthologous residues
CCDC27 (human): S149‑p, CCDC27 (mouse): S107‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S124-p - DNAI1 (mouse)
Modsite: RDEMVAGsQEsIKVV SwissProt Entrez-Gene
Orthologous residues
DNAI1 (human): S131‑p, DNAI1 (mouse): S124‑p, DNAI1 (rat): S124‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S127-p - DNAI1 (mouse)
Modsite: MVAGsQEsIKVVTSE SwissProt Entrez-Gene
Orthologous residues
DNAI1 (human): S134‑p, DNAI1 (mouse): S127‑p, DNAI1 (rat): S127‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S13-p - FABP9 (mouse)
Modsite: LGTWKLIsSENFENY SwissProt Entrez-Gene
Orthologous residues
FABP9 (human): S13‑p, FABP9 (mouse): S13‑p, FABP9 (rat): S13‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S449-p - FAM154A (mouse)
Modsite: HKIYRSIsQTGsRQN SwissProt Entrez-Gene
Orthologous residues
FAM154A (human): S447‑p, FAM154A (mouse): S449‑p, FAM154A (rat): S447‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S453-p - FAM154A (mouse)
Modsite: RSIsQTGsRQNSSIS SwissProt Entrez-Gene
Orthologous residues
FAM154A (human): S451‑p, FAM154A (mouse): S453‑p, FAM154A (rat): S451‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S168-p - FAM166C (mouse)
Modsite: KWNLLRLsPDYKRTY SwissProt Entrez-Gene
Orthologous residues
FAM166C (human): A169‑p, FAM166C (mouse): S168‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S5-p - GSTM3 (mouse)
Modsite: ___MSSksMVLGYWD SwissProt Entrez-Gene
Orthologous residues
GSTM3 (human): S6‑p, GSTM3 (mouse): S5‑p, GSTM3 (rat): S6‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

Y83-p - HK1 (mouse)
Modsite: EKIDKYLyAMRLSDE SwissProt Entrez-Gene
Orthologous residues
HK1 (human): Y27‑p, HK1 iso2 (human): Y26‑p, HK1 iso3 (human): Y31‑p, HK1 (mouse): Y83‑p, HK1 iso3 (mouse): Y26‑p, HK1 (rat): Y27‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S464-p - MKK7 (mouse)
Modsite: RCRGRVPsPRRATGG SwissProt Entrez-Gene
Orthologous residues
MKK7 (human): , MKK7 iso2 (human): , MKK7 iso3 (human): , MKK7 (mouse): S464‑p, MKK7 iso2 (mouse): , MKK7 iso6 (mouse): , MKK7 (rat):

S43-p - MLF1 (mouse)
Modsite: PLGRDLLsIsDGrGR SwissProt Entrez-Gene
Orthologous residues
MLF1 (human): S43‑p, MLF1 iso2 (human): S18‑p, MLF1 iso3 (human): S59‑p, MLF1 (mouse): S43‑p, MLF1 (rat): S43‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S45-p - MLF1 (mouse)
Modsite: GRDLLsIsDGrGRTH SwissProt Entrez-Gene
Orthologous residues
MLF1 (human): S45‑p, MLF1 iso2 (human): S20‑p, MLF1 iso3 (human): S61‑p, MLF1 (mouse): S45‑p, MLF1 (rat): S45‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

Y18-p - NT5C1B (mouse)
Modsite: KNEPGMRysKEsLDA SwissProt Entrez-Gene
Orthologous residues
NT5C1B (human): S17‑p, NT5C1B iso2 (human): S17‑p, NT5C1B iso5 (human): S17‑p, NT5C1B (mouse): Y18‑p, NT5C1B (rat): Y18‑p

S19-p - NT5C1B (mouse)
Modsite: NEPGMRysKEsLDAE SwissProt Entrez-Gene
Orthologous residues
NT5C1B (human): S18‑p, NT5C1B iso2 (human): S18‑p, NT5C1B iso5 (human): S18‑p, NT5C1B (mouse): S19‑p, NT5C1B (rat): S19‑p

S22-p - NT5C1B (mouse)
Modsite: GMRysKEsLDAEKRK SwissProt Entrez-Gene
Orthologous residues
NT5C1B (human): S21‑p, NT5C1B iso2 (human): S21‑p, NT5C1B iso5 (human): S21‑p, NT5C1B (mouse): S22‑p, NT5C1B (rat): S22‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S632-p - ODF2 (mouse)
Modsite: IDLTAIIsDLRSRIE SwissProt Entrez-Gene
Orthologous residues
ODF2 (human): S632‑p, ODF2 iso3 (human): S608‑p, ODF2 (mouse): S632‑p, ODF2 iso4 (mouse): S628‑p, ODF2 (rat): S627‑p, ODF2 iso3 (rat): S613‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S222-p - ODF3 (mouse)
Modsite: KPGPGAHsPEKVTLN SwissProt Entrez-Gene
Orthologous residues
ODF3 (human): S222‑p, ODF3 iso2 (human): , ODF3 (mouse): S222‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S361-p - RGS22 (mouse)
Modsite: PSNKAIVsPPVEMVE SwissProt Entrez-Gene
Orthologous residues
RGS22 (human): S367‑p, RGS22 (mouse): S361‑p, RGS22 (rat): S246‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation decrease

S371-p - RGS22 (mouse)
Modsite: VEMVEEIsKDRLENV SwissProt Entrez-Gene
Orthologous residues
RGS22 (human): S377‑p, RGS22 (mouse): S371‑p, RGS22 (rat): S256‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation decrease

S57-p - RPS27A (mouse)
Modsite: LEDGRTLsDYNIQkE SwissProt Entrez-Gene
Orthologous residues
RPS27A (human): S57‑p, RPS27A (mouse): S57‑p, RPS27A (rat): S57‑p, RPS27A (cow): S57‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  ambiguous site (within peptides)

S56-p - Slco6c1 (mouse)
Modsite: KLPKRKKsPTsAELL SwissProt Entrez-Gene
Orthologous residues
Slco6c1 (mouse): S56‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S59-p - Slco6c1 (mouse)
Modsite: KRKKsPTsAELLLID SwissProt Entrez-Gene
Orthologous residues
Slco6c1 (mouse): S59‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

T265-p - SPACA1 (mouse)
Modsite: KSFWGSKtsAtEIQs SwissProt Entrez-Gene
Orthologous residues
SPACA1 (human): A255‑p, SPACA1 (mouse): T265‑p, SPACA1 (rat): A260‑p

S266-p - SPACA1 (mouse)
Modsite: SFWGSKtsAtEIQsE SwissProt Entrez-Gene
Orthologous residues
SPACA1 (human): S256‑p, SPACA1 (mouse): S266‑p, SPACA1 (rat): S261‑p

T268-p - SPACA1 (mouse)
Modsite: WGSKtsAtEIQsELs SwissProt Entrez-Gene
Orthologous residues
SPACA1 (human): P258‑p, SPACA1 (mouse): T268‑p, SPACA1 (rat): T263‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S272-p - SPACA1 (mouse)
Modsite: tsAtEIQsELssMRY SwissProt Entrez-Gene
Orthologous residues
SPACA1 (human): S262‑p, SPACA1 (mouse): S272‑p, SPACA1 (rat): S267‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S275-p - SPACA1 (mouse)
Modsite: tEIQsELssMRYKDS SwissProt Entrez-Gene
Orthologous residues
SPACA1 (human): S265‑p, SPACA1 (mouse): S275‑p, SPACA1 (rat): S270‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S276-p - SPACA1 (mouse)
Modsite: EIQsELssMRYKDST SwissProt Entrez-Gene
Orthologous residues
SPACA1 (human): S266‑p, SPACA1 (mouse): S276‑p, SPACA1 (rat): S271‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S283-p - SPATA18 (mouse)
Modsite: TTVAKIRsPsPNRAK SwissProt Entrez-Gene
Orthologous residues
SPATA18 (human): R285‑p, SPATA18 (mouse): S283‑p, SPATA18 (rat): S303‑p, SPATA18 iso2 (rat): S303‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  ambiguous site (within proteins)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S285-p - SPATA18 (mouse)
Modsite: VAKIRsPsPNRAKMS SwissProt Entrez-Gene
Orthologous residues
SPATA18 (human): S287‑p, SPATA18 (mouse): S285‑p, SPATA18 (rat): S305‑p, SPATA18 iso2 (rat): S305‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  ambiguous site (within proteins)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S526-p - SPATA18 (mouse)
Modsite: STVSRSRsPsPIRCT SwissProt Entrez-Gene
Orthologous residues
SPATA18 (human): S527‑p, SPATA18 (mouse): S526‑p, SPATA18 (rat): S557‑p, SPATA18 iso2 (rat): S545‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S528-p - SPATA18 (mouse)
Modsite: VSRSRsPsPIRCTFA SwissProt Entrez-Gene
Orthologous residues
SPATA18 (human): S529‑p, SPATA18 (mouse): S528‑p, SPATA18 (rat): S559‑p, SPATA18 iso2 (rat): S547‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S72-p - ST6GALNAC2 iso2 (mouse)
Modsite: SQPSSRAssQsPsPH SwissProt Entrez-Gene
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S72‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S73-p - ST6GALNAC2 iso2 (mouse)
Modsite: QPSSRAssQsPsPHV SwissProt Entrez-Gene
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S73‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S75-p - ST6GALNAC2 iso2 (mouse)
Modsite: SSRAssQsPsPHVQH SwissProt Entrez-Gene
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S75‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S77-p - ST6GALNAC2 iso2 (mouse)
Modsite: RAssQsPsPHVQHVP SwissProt Entrez-Gene
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S77‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S100-p - ST6GALNAC2 iso2 (mouse)
Modsite: PPASKSPsQTGLKAL SwissProt Entrez-Gene
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S100‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  ambiguous site (within peptides)

S122-p - ST6GALNAC2 iso2 (mouse)
Modsite: PPVNITRsPsHsPAT SwissProt Entrez-Gene
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S122‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S124-p - ST6GALNAC2 iso2 (mouse)
Modsite: VNITRsPsHsPATsA SwissProt Entrez-Gene
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S124‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S126-p - ST6GALNAC2 iso2 (mouse)
Modsite: ITRsPsHsPATsAsY SwissProt Entrez-Gene
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S126‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S130-p - ST6GALNAC2 iso2 (mouse)
Modsite: PsHsPATsAsYIGPI SwissProt Entrez-Gene
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S130‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S132-p - ST6GALNAC2 iso2 (mouse)
Modsite: HsPATsAsYIGPIRN SwissProt Entrez-Gene
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S132‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S271-p - ST6GALNAC2 iso2 (mouse)
Modsite: HRRLTLPsLSLQYDG SwissProt Entrez-Gene
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S271‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S18-p - TCTE3 (mouse)
Modsite: PTGQTHQsPVSkRER SwissProt Entrez-Gene
Orthologous residues
TCTE3 (human): A23‑p, TCTE3 (mouse): S18‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S10-p - THEG (mouse)
Modsite: ELGEHRAsLLsNPIP SwissProt Entrez-Gene
Orthologous residues
THEG (human): S8‑p, THEG (mouse): S10‑p, THEG (rat): S12‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S13-p - THEG (mouse)
Modsite: EHRAsLLsNPIPEVK SwissProt Entrez-Gene
Orthologous residues
THEG (human): N11‑p, THEG (mouse): S13‑p, THEG (rat): S15‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S57-p - UBA52 (mouse)
Modsite: LEDGRTLsDYNIQkE SwissProt Entrez-Gene
Orthologous residues
UBA52 (human): S57‑p, UBA52 (mouse): S57‑p, UBA52 (rat): S57‑p, UBA52 (cow): S57‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  ambiguous site (between proteins)

S57-p - UBB (mouse)
Modsite: LEDGRTLsDYNIQkE SwissProt Entrez-Gene
Orthologous residues
UBB (human): S57‑p, UBB (mouse): S57‑p, UBB (rat): S57‑p, UBB (cow): S57‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse

S133-p - UBB (mouse)
Modsite: LEDGRTLsDYNIQkE SwissProt Entrez-Gene
Orthologous residues
UBB (human): S133‑p, UBB (mouse): S133‑p, UBB (rat): S133‑p, UBB (cow): S133‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  ambiguous site (within peptides)

S209-p - UBB (mouse)
Modsite: LEDGRTLsDYNIQkE SwissProt Entrez-Gene
Orthologous residues
UBB (human): S209‑p, UBB (mouse): S209‑p, UBB (rat): S209‑p, UBB (cow): S209‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  ambiguous site (within peptides)

S285-p - UBB (mouse)
Modsite: LEDGRTLsDYNIQkE SwissProt Entrez-Gene
Orthologous residues
UBB (human): , UBB (mouse): S285‑p, UBB (rat): S285‑p, UBB (cow): S285‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  ambiguous site (within peptides)

S57-p - UBC (mouse)
Modsite: LEDGRTLsDYNIQkE SwissProt Entrez-Gene
Orthologous residues
UBC (human): S57‑p, UBC (mouse): S57‑p, UBC iso100 (mouse): S57‑p, UBC (rat): S57‑p, UBC (cow): S57‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  ambiguous site (between proteins and within proteins)

S133-p - UBC (mouse)
Modsite: LEDGRTLsDYNIQkE SwissProt Entrez-Gene
Orthologous residues
UBC (human): S133‑p, UBC (mouse): S133‑p, UBC iso100 (mouse): S133‑p, UBC (rat): S133‑p, UBC (cow): S133‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  ambiguous site (between proteins and within proteins)

S209-p - UBC (mouse)
Modsite: LEDGRTLsDYNIQkE SwissProt Entrez-Gene
Orthologous residues
UBC (human): S209‑p, UBC (mouse): S209‑p, UBC iso100 (mouse): S209‑p, UBC (rat): S209‑p, UBC (cow): S209‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  ambiguous site (between proteins and within proteins)

S285-p - UBC (mouse)
Modsite: LEDGRTLsDYNIQkE SwissProt Entrez-Gene
Orthologous residues
UBC (human): S285‑p, UBC (mouse): S285‑p, UBC iso100 (mouse): S285‑p, UBC (rat): S285‑p, UBC (cow): S285‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  ambiguous site (between proteins and within proteins)

S361-p - UBC (mouse)
Modsite: LEDGRTLsDYNIQkE SwissProt Entrez-Gene
Orthologous residues
UBC (human): S361‑p, UBC (mouse): S361‑p, UBC iso100 (mouse): S361‑p, UBC (rat): S361‑p, UBC (cow): S361‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  ambiguous site (between proteins and within proteins)

S437-p - UBC (mouse)
Modsite: LEDGRTLsDYNIQkE SwissProt Entrez-Gene
Orthologous residues
UBC (human): S437‑p, UBC (mouse): S437‑p, UBC iso100 (mouse): S437‑p, UBC (rat): S437‑p, UBC (cow): S437‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  ambiguous site (between proteins and within proteins)

S513-p - UBC (mouse)
Modsite: LEDGRTLsDYNIQkE SwissProt Entrez-Gene
Orthologous residues
UBC (human): S513‑p, UBC (mouse): S513‑p, UBC iso100 (mouse): S513‑p, UBC (rat): S513‑p, UBC (cow): S513‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  ambiguous site (between proteins and within proteins)

S589-p - UBC (mouse)
Modsite: LEDGRTLsDYNIQkE SwissProt Entrez-Gene
Orthologous residues
UBC (human): S589‑p, UBC (mouse): S589‑p, UBC iso100 (mouse): S589‑p, UBC (rat): S589‑p, UBC (cow): S589‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  ambiguous site (between proteins and within proteins)

S665-p - UBC (mouse)
Modsite: LEDGRTLsDYNIQkE SwissProt Entrez-Gene
Orthologous residues
UBC (human): S665‑p, UBC (mouse): S665‑p, UBC iso100 (mouse): S665‑p, UBC (rat): S665‑p, UBC (cow): S665‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  ambiguous site (between proteins and within proteins)

S57-p - ubiquitin (mouse)
Modsite: LEDGRTLsDYNIQkE SwissProt
Orthologous residues
ubiquitin (human): S57‑p, ubiquitin (mouse): S57‑p, ubiquitin (rat): S57‑p, ubiquitin (cow): S57‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  ambiguous site (between proteins)

S958-p - WDR64 (mouse)
Modsite: RERWKKMssMSLLFK SwissProt Entrez-Gene
Orthologous residues
WDR64 (human): S959‑p, WDR64 (mouse): S958‑p, WDR64 (rat): S969‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase

S959-p - WDR64 (mouse)
Modsite: ERWKKMssMSLLFKR SwissProt Entrez-Gene
Orthologous residues
WDR64 (human): S960‑p, WDR64 (mouse): S959‑p, WDR64 (rat): S970‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  spermatozoa
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm_capacitation increase