Curated Information
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Home > Curated Information Page > PubMed Id: 29921588
Murillo-de-Ozores AR, et al. (2018) C-terminally truncated, kidney-specific variants of the WNK4 kinase lack several sites that regulate its activity. J Biol Chem 29921588
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S382-p - STLK3 (mouse)
Modsite: KVRRVPGssGHLHKT SwissProt Entrez-Gene
Orthologous residues
STLK3 (human): S370‑p, STLK3 (mouse): S382‑p, STLK3 (rat): S379‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE WNK4 (human) transfection of inactive enzyme, transfection of constitutively active enzyme, transfection of wild-type enzyme

S332-p - WNK4 (mouse)
Modsite: kRAsFAKsVIGTPEF SwissProt Entrez-Gene
Orthologous residues
WNK4 (human): S335‑p, WNK4 (mouse): S332‑p, WNK4 (rat): S332‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP1CA (human) transfection of inactive enzyme, transfection of wild-type enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  phosphorylation
Comments:  induces phosphorylation of STLK3