Curated Information
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Home > Curated Information Page > PubMed Id: 15073328
Shi Y, et al. (2004) Direct regulation of CREB transcriptional activity by ATM in response to genotoxic stress. Proc Natl Acad Sci U S A 101, 5898-903 15073328
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S97-p - CREB (human)
Modsite: TIAEsEDsQEsVDsV SwissProt Entrez-Gene
Orthologous residues
CREB (human): S97‑p, CREB iso1 (human): S111‑p, CREB (mouse): S97‑p, CREB iso1 (mouse): S111‑p, CREB (rat): S111‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  phosphorylation of this site primes phosphorylation of S121 in response to the stress
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ATM (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
Downstream Regulation
Effect of modification (function):  activity, inhibited
Effect of modification (process):  transcription, inhibited

S107-p - CREB (human)
Modsite: sVDsVtDsQKRREIL SwissProt Entrez-Gene
Orthologous residues
CREB (human): S107‑p, CREB iso1 (human): S121‑p, CREB (mouse): S107‑p, CREB iso1 (mouse): S121‑p, CREB (rat): S121‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  leukemia, chronic myelogenous leukemia
Relevant cell lines - cell types - tissues:  293 (epithelial), K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ATM (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) genetic knockout/knockin of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
ionizing_radiation increase
UV increase
aphidicolin increase
deferoxamine increase
Downstream Regulation
Effect of modification (function):  activity, inhibited
Effect of modification (process):  transcription, inhibited

S119-p - CREB (human)
Modsite: EILsRRPsyRkILND SwissProt Entrez-Gene
Orthologous residues
CREB (human): S119‑p, CREB iso1 (human): S133‑p, CREB (mouse): S119‑p, CREB iso1 (mouse): S133‑p, CREB (rat): S133‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
H2O2 increase
Downstream Regulation
Effect of modification (function):  activity, induced
Effect of modification (process):  transcription, induced

T100-p - CREB iso1 (human)
Modsite: LKRLFsGtQISTIAE SwissProt Entrez-Gene
Orthologous residues
CREB (human): , CREB iso1 (human): T100‑p, CREB (mouse): , CREB iso1 (mouse): T100‑p, CREB (rat): T100‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ATM (human)
Downstream Regulation
Effect of modification (function):  activity, inhibited
Effect of modification (process):  transcription, inhibited

S107-p - CREB (mouse)
Modsite: sVDsVTDsQKRREIL SwissProt Entrez-Gene
Orthologous residues
CREB (human): S107‑p, CREB iso1 (human): S121‑p, CREB (mouse): S107‑p, CREB iso1 (mouse): S121‑p, CREB (rat): S121‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
wortmannin ionizing_radiation inhibit treatment-induced increase