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Home > Curated Information Page > PubMed Id: 15647351
Ballif BA, et al. (2005) Quantitative phosphorylation profiling of the ERK/p90 ribosomal S6 kinase-signaling cassette and its targets, the tuberous sclerosis tumor suppressors. Proc Natl Acad Sci U S A 102, 667-72 15647351
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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T185-p - ERK2 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y187-p - ERK2 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S221-p - p90RSK (human)
Modsite: DHEKKAysFCGtVEy SwissProt Entrez-Gene
Orthologous residues
p90RSK (human): S221‑p, p90RSK iso2 (human): S230‑p, p90RSK (mouse): S221‑p, p90RSK iso3 (mouse): S227‑p, p90RSK (rat): S221‑p, p90RSK (chicken): S239‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

T359-p - p90RSK (human)
Modsite: DTEFTSRtPKDsPGI SwissProt Entrez-Gene
Orthologous residues
p90RSK (human): T359‑p, p90RSK iso2 (human): T368‑p, p90RSK (mouse): T348‑p, p90RSK iso3 (mouse): , p90RSK (rat): T359‑p, p90RSK (chicken): T377‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S363-p - p90RSK (human)
Modsite: TSRtPKDsPGIPPsA SwissProt Entrez-Gene
Orthologous residues
p90RSK (human): S363‑p, p90RSK iso2 (human): S372‑p, p90RSK (mouse): S352‑p, p90RSK iso3 (mouse): , p90RSK (rat): S363‑p, p90RSK (chicken): S381‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S380-p - p90RSK (human)
Modsite: HQLFRGFsFVAtGLM SwissProt Entrez-Gene
Orthologous residues
p90RSK (human): S380‑p, p90RSK iso2 (human): S389‑p, p90RSK (mouse): S369‑p, p90RSK iso3 (mouse): , p90RSK (rat): S380‑p, p90RSK (chicken): S398‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

T573-p - p90RSK (human)
Modsite: AENGLLMtPCytANF SwissProt Entrez-Gene
Orthologous residues
p90RSK (human): T573‑p, p90RSK iso2 (human): T582‑p, p90RSK (mouse): T562‑p, p90RSK iso3 (mouse): , p90RSK (rat): T573‑p, p90RSK (chicken): T590‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S732-p - p90RSK (human)
Modsite: RRVRKLPsttL____ SwissProt Entrez-Gene
Orthologous residues
p90RSK (human): S732‑p, p90RSK iso2 (human): S741‑p, p90RSK (mouse): S721‑p, p90RSK iso3 (mouse): , p90RSK (rat): S732‑p, p90RSK (chicken): S749‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S664-p - TSC2 (human)
Modsite: KkTSGPLsPPTGPPG SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S664‑p, TSC2 iso2 (human): S664‑p, TSC2 iso3 (human): S664‑p, TSC2 iso4 (human): S664‑p, TSC2 (mouse): S664‑p, TSC2 iso6 (mouse): S664‑p, TSC2 (rat): S664‑p, TSC2 iso2 (rat): S664‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase

S960-p - TSC2 (human)
Modsite: PkQGLNNsPPVkEFk SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S960‑p, TSC2 iso2 (human): , TSC2 iso3 (human): , TSC2 iso4 (human): S960‑p, TSC2 (mouse): S960‑p, TSC2 iso6 (mouse): S960‑p, TSC2 (rat): S960‑p, TSC2 iso2 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase

S981-p - TSC2 (human)
Modsite: AFRCRSIsVSEHVVR SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S981‑p, TSC2 iso2 (human): , TSC2 iso3 (human): , TSC2 iso4 (human): S981‑p, TSC2 (mouse): S981‑p, TSC2 iso6 (mouse): S981‑p, TSC2 (rat): S981‑p, TSC2 iso2 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase

S1096-p - TSC2 (human)
Modsite: QSGPESSssPGVHVR SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1096‑p, TSC2 iso2 (human): S1053‑p, TSC2 iso3 (human): S1052‑p, TSC2 iso4 (human): S1096‑p, TSC2 (mouse): S1096‑p, TSC2 iso6 (mouse): S1096‑p, TSC2 (rat): S1096‑p, TSC2 iso2 (rat): S1053‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase

S1097-p - TSC2 (human)
Modsite: SGPESSssPGVHVRQ SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1097‑p, TSC2 iso2 (human): S1054‑p, TSC2 iso3 (human): S1053‑p, TSC2 iso4 (human): S1097‑p, TSC2 (mouse): D1097‑p, TSC2 iso6 (mouse): D1097‑p, TSC2 (rat): D1097‑p, TSC2 iso2 (rat): D1054‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase

S1132-p - TSC2 (human)
Modsite: RDRVRsMsGGHGLRV SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1132‑p, TSC2 iso2 (human): S1089‑p, TSC2 iso3 (human): S1088‑p, TSC2 iso4 (human): S1132‑p, TSC2 (mouse): S1132‑p, TSC2 iso6 (mouse): S1132‑p, TSC2 (rat): S1132‑p, TSC2 iso2 (rat): S1089‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester no change compared to control

S1155-p - TSC2 (human)
Modsite: QFLGSATsPGPRTAP SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1155‑p, TSC2 iso2 (human): S1112‑p, TSC2 iso3 (human): S1111‑p, TSC2 iso4 (human): S1155‑p, TSC2 (mouse): S1155‑p, TSC2 iso6 (mouse): S1155‑p, TSC2 (rat): S1155‑p, TSC2 iso2 (rat): S1112‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase

S1364-p - TSC2 (human)
Modsite: IPIERVVssEGGRPs SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1364‑p, TSC2 iso2 (human): S1321‑p, TSC2 iso3 (human): S1320‑p, TSC2 iso4 (human): S1341‑p, TSC2 (mouse): S1365‑p, TSC2 iso6 (mouse): S1343‑p, TSC2 (rat): S1366‑p, TSC2 iso2 (rat): S1323‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
bisindolylmaleimide phorbol_ester inhibit treatment-induced increase
U0126 phorbol_ester inhibit treatment-induced increase
phorbol_ester increase

S1371-p - TSC2 (human)
Modsite: ssEGGRPsVDLsFQP SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1371‑p, TSC2 iso2 (human): S1328‑p, TSC2 iso3 (human): S1327‑p, TSC2 iso4 (human): S1348‑p, TSC2 (mouse): A1372‑p, TSC2 iso6 (mouse): A1350‑p, TSC2 (rat): T1373‑p, TSC2 iso2 (rat): T1330‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester decrease

S1385-p - TSC2 (human)
Modsite: PsQPLsKssssPELQ SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1385‑p, TSC2 iso2 (human): S1342‑p, TSC2 iso3 (human): S1341‑p, TSC2 iso4 (human): S1362‑p, TSC2 (mouse): S1386‑p, TSC2 iso6 (mouse): S1364‑p, TSC2 (rat): S1387‑p, TSC2 iso2 (rat): S1344‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester decrease

S1386-p - TSC2 (human)
Modsite: sQPLsKssssPELQt SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1386‑p, TSC2 iso2 (human): S1343‑p, TSC2 iso3 (human): S1342‑p, TSC2 iso4 (human): S1363‑p, TSC2 (mouse): S1387‑p, TSC2 iso6 (mouse): S1365‑p, TSC2 (rat): S1388‑p, TSC2 iso2 (rat): S1345‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester decrease

S1387-p - TSC2 (human)
Modsite: QPLsKssssPELQtL SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1387‑p, TSC2 iso2 (human): S1344‑p, TSC2 iso3 (human): S1343‑p, TSC2 iso4 (human): S1364‑p, TSC2 (mouse): S1388‑p, TSC2 iso6 (mouse): S1366‑p, TSC2 (rat): S1389‑p, TSC2 iso2 (rat): S1346‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester decrease

S1388-p - TSC2 (human)
Modsite: PLsKssssPELQtLQ SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1388‑p, TSC2 iso2 (human): S1345‑p, TSC2 iso3 (human): S1344‑p, TSC2 iso4 (human): S1365‑p, TSC2 (mouse): S1389‑p, TSC2 iso6 (mouse): S1367‑p, TSC2 (rat): S1390‑p, TSC2 iso2 (rat): S1347‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester decrease

S1411-p - TSC2 (human)
Modsite: KADVGRLsPEVKARs SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1411‑p, TSC2 iso2 (human): S1368‑p, TSC2 iso3 (human): S1367‑p, TSC2 iso4 (human): S1388‑p, TSC2 (mouse): S1412‑p, TSC2 iso6 (mouse): S1390‑p, TSC2 (rat): S1413‑p, TSC2 iso2 (rat): S1370‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase

S1418-p - TSC2 (human)
Modsite: sPEVKARsQsGtLDG SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1418‑p, TSC2 iso2 (human): S1375‑p, TSC2 iso3 (human): S1374‑p, TSC2 iso4 (human): S1395‑p, TSC2 (mouse): S1419‑p, TSC2 iso6 (mouse): S1397‑p, TSC2 (rat): S1420‑p, TSC2 iso2 (rat): S1377‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase

S1420-p - TSC2 (human)
Modsite: EVKARsQsGtLDGEs SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1420‑p, TSC2 iso2 (human): S1377‑p, TSC2 iso3 (human): S1376‑p, TSC2 iso4 (human): S1397‑p, TSC2 (mouse): S1421‑p, TSC2 iso6 (mouse): S1399‑p, TSC2 (rat): S1422‑p, TSC2 iso2 (rat): S1379‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase

S1798-p - TSC2 (human)
Modsite: GQRKRLIssVEDFTE SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1798‑p, TSC2 iso2 (human): S1755‑p, TSC2 iso3 (human): S1754‑p, TSC2 iso4 (human): S1775‑p, TSC2 (mouse): S1805‑p, TSC2 iso6 (mouse): S1777‑p, TSC2 (rat): S1800‑p, TSC2 iso2 (rat): S1757‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293E (epithelial)
Cellular systems studied:  cell lines
Species studied:  human