Curated Information
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Home > Curated Information Page > PubMed Id: 11401470
Tennagels N, et al. (2001) Identification of Ser(1275) and Ser(1309) as autophosphorylation sites of the human insulin receptor in intact cells. Biochem Biophys Res Commun 282, 387-93 11401470
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y1173-p - INSR iso2 (human)
Modsite: FGMTRDIyETDyyRK SwissProt Entrez-Gene
Orthologous residues
INSR (human): Y1185‑p, INSR iso2 (human): Y1173‑p, INSR (mouse): Y1175‑p, INSR (rat): Y1186‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, peptide sequencing, phospho-antibody, phosphoamino acid analysis
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], CHO (fibroblast) [EphB1 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE INSR (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

Y1177-p - INSR iso2 (human)
Modsite: RDIyETDyyRKGGKG SwissProt Entrez-Gene
Orthologous residues
INSR (human): Y1189‑p, INSR iso2 (human): Y1177‑p, INSR (mouse): Y1179‑p, INSR (rat): Y1190‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, peptide sequencing, phospho-antibody, phosphoamino acid analysis
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], CHO (fibroblast) [EphB1 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE INSR (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

Y1178-p - INSR iso2 (human)
Modsite: DIyETDyyRKGGKGL SwissProt Entrez-Gene
Orthologous residues
INSR (human): Y1190‑p, INSR iso2 (human): Y1178‑p, INSR (mouse): Y1180‑p, INSR (rat): Y1191‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, peptide sequencing, phospho-antibody, phosphoamino acid analysis
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], CHO (fibroblast) [EphB1 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE INSR (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

S1302-p - INSR iso2 (human)
Modsite: EENKAPEsEELEMEF SwissProt Entrez-Gene
Orthologous residues
INSR (human): S1314‑p, INSR iso2 (human): S1302‑p, INSR (mouse): S1304‑p, INSR (rat): S1315‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, peptide sequencing, phospho-antibody, phosphoamino acid analysis
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], CHO (fibroblast) [EphB1 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE INSR (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

S1336-p - INSR iso2 (human)
Modsite: GGRDGGSsLGFKRSy SwissProt Entrez-Gene
Orthologous residues
INSR (human): S1348‑p, INSR iso2 (human): S1336‑p, INSR (mouse): S1338‑p, INSR (rat): S1349‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, peptide sequencing, phospho-antibody, phosphoamino acid analysis
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], CHO (fibroblast) [EphB1 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE INSR (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

Y1343-p - INSR iso2 (human)
Modsite: sLGFKRSyEEHIPyT SwissProt Entrez-Gene
Orthologous residues
INSR (human): Y1355‑p, INSR iso2 (human): Y1343‑p, INSR (mouse): Y1345‑p, INSR (rat): Y1356‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, peptide sequencing, phospho-antibody, phosphoamino acid analysis
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], CHO (fibroblast) [EphB1 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE INSR (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

Y1349-p - INSR iso2 (human)
Modsite: SyEEHIPyTHMNGGK SwissProt Entrez-Gene
Orthologous residues
INSR (human): Y1361‑p, INSR iso2 (human): Y1349‑p, INSR (mouse): Y1351‑p, INSR (rat): Y1362‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, peptide sequencing, phospho-antibody, phosphoamino acid analysis
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], CHO (fibroblast) [EphB1 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE INSR (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase