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Home > Curated Information Page > PubMed Id: 19060905
Lorenz K, Schmitt JP, Schmitteckert EM, Lohse MJ (2009) A new type of ERK1/2 autophosphorylation causes cardiac hypertrophy. Nat Med 15, 75-83 19060905
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T202-p - ERK1 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
heart failure increase

Y204-p - ERK1 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
heart failure increase

T185-p - ERK2 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
heart failure increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, molecular association, regulation, protein conformation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
G-beta 1 (human) Induces co-immunoprecipitation

Y187-p - ERK2 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
heart failure increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, molecular association, regulation, protein conformation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
G-beta 1 (human) Induces co-immunoprecipitation

T190-p - ERK2 (human)
Modsite: FLtEyVAtRWyrAPE SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T190‑p, ERK2 (mouse): T188‑p, ERK2 (rat): T188‑p, ERK2 (chicken): T198‑p, ERK2 (cow): T190‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
heart failure increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, molecular association, regulation, protein conformation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
G-beta 1 (human) Induces co-immunoprecipitation
Associated Diseases
Diseases Alterations Comments
ventricular tachycardia, hypertrophic cardiomyopathy increased

S384-p - ELK1 (mouse)
Modsite: IHFWSTLsPIAPRsP SwissProt Entrez-Gene
Orthologous residues
ELK1 (human): S383‑p, ELK1 (mouse): S384‑p, ELK1 (rat): S382‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ERK2 (rat) increase T188 phosphorylation increases ERK phosphorylation of nuclear targets

T203-p - ERK1 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p

Y205-p - ERK1 (mouse)
Modsite: HtGFLtEyVAtRWyR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p

T183-p - ERK2 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p

Y185-p - ERK2 (mouse)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p

T188-p - ERK2 (mouse)
Modsite: FLtEyVAtRWYRAPE SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T190‑p, ERK2 (mouse): T188‑p, ERK2 (rat): T188‑p, ERK2 (chicken): T198‑p, ERK2 (cow): T190‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
heart failure increase
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  cell growth, altered, cytoskeletal reorganization
Comments:  T 188 phosphorylation induces ERK nuclear localization and cardiac hypertrophy.
Associated Diseases
Diseases Alterations Comments
ventricular tachycardia, hypertrophic cardiomyopathy increased

T645-p - MSK1 (mouse)
Modsite: PDNQPLKtPCFTLHY SwissProt Entrez-Gene
Orthologous residues
MSK1 (human): T581‑p, MSK1 (mouse): T645‑p, MSK1 (rat):
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ERK2 (rat) increase T188 phosphorylation increases ERK phosphorylation of nuclear targets

T58-p - Myc (mouse)
Modsite: KKFELLPtPPLsPsR SwissProt Entrez-Gene
Orthologous residues
Myc (human): T58‑p, Myc iso2 (human): T73‑p, Myc (mouse): T58‑p, Myc (rat): T58‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ERK2 (rat) increase T188 phosphorylation increases ERK phosphorylation of nuclear targets

S62-p - Myc (mouse)
Modsite: LLPtPPLsPsRRsGL SwissProt Entrez-Gene
Orthologous residues
Myc (human): S62‑p, Myc iso2 (human): S77‑p, Myc (mouse): S62‑p, Myc (rat): S62‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ERK2 (rat) increase T188 phosphorylation increases ERK phosphorylation of nuclear targets

T444-p - p70S6K (mouse)
Modsite: RFIGsPRtPVsPVKF SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T444‑p, p70S6K iso2 (human): T421‑p, p70S6K (mouse): T444‑p, p70S6K (rat): T444‑p, p70S6K iso2 (rat): T421‑p, p70S6K (fruit fly):
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
pressure increase transverse aortic constriction (TAC)

S447-p - p70S6K (mouse)
Modsite: GsPRtPVsPVKFsPG SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): S447‑p, p70S6K iso2 (human): S424‑p, p70S6K (mouse): S447‑p, p70S6K (rat): S447‑p, p70S6K iso2 (rat): S424‑p, p70S6K (fruit fly):
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
pressure increase transverse aortic constriction (TAC)

S369-p - p90RSK (mouse)
Modsite: HQLFRGFsFVATGLM SwissProt Entrez-Gene
Orthologous residues
p90RSK (human): S380‑p, p90RSK iso2 (human): S389‑p, p90RSK (mouse): S369‑p, p90RSK iso3 (mouse): , p90RSK (rat): S380‑p, p90RSK (chicken): S398‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
pressure increase transverse aortic constriction (TAC)

T203-p - ERK1 (rat)
Modsite: HDHTGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), heart, myocyte-heart
Cellular systems studied:  primary cells, tissue
Species studied:  human, mouse, rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE MEK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
carbachol increase long-term stimulation
angiotensin_2 increase
neuregulin increase
phenylephrine increase
carbachol increase short-term stimulation
angiotensin_2 increase
neuregulin increase
phenylephrine increase

Y205-p - ERK1 (rat)
Modsite: HTGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), heart, myocyte-heart
Cellular systems studied:  primary cells, tissue
Species studied:  human, mouse, rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE MEK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
carbachol increase long-term stimulation
angiotensin_2 increase
neuregulin increase
phenylephrine increase
carbachol increase short-term stimulation
angiotensin_2 increase
neuregulin increase
phenylephrine increase

T183-p - ERK2 (rat)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), heart, myocyte-heart
Cellular systems studied:  primary cells, tissue
Species studied:  human, mouse, rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE MEK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
carbachol increase long-term stimulation
angiotensin_2 increase
neuregulin increase
phenylephrine increase
carbachol increase short-term stimulation
angiotensin_2 increase
neuregulin increase
phenylephrine increase

Y185-p - ERK2 (rat)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), heart, myocyte-heart
Cellular systems studied:  primary cells, tissue
Species studied:  human, mouse, rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE MEK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
carbachol increase long-term stimulation
angiotensin_2 increase
neuregulin increase
phenylephrine increase
carbachol increase short-term stimulation
angiotensin_2 increase
neuregulin increase
phenylephrine increase

T188-p - ERK2 (rat)
Modsite: FLtEyVAtRWYRAPE SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T190‑p, ERK2 (mouse): T188‑p, ERK2 (rat): T188‑p, ERK2 (chicken): T198‑p, ERK2 (cow): T190‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), heart, myocyte-heart
Cellular systems studied:  primary cells, tissue
Species studied:  human, mouse, rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (rat)
Comments:  autophosphorylation induced by G-beta-gamma
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
carbachol no change compared to control long-term stimulation
angiotensin_2 increase
neuregulin increase
phenylephrine increase
carbachol no change compared to control short-term stimulation
angiotensin_2 increase
neuregulin increase
phenylephrine increase