Curated Information
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Home > Curated Information Page > PubMed Id: 19047162
Koomoa DL, et al. (2008) Ornithine decarboxylase inhibition by alpha-difluoromethylornithine activates opposing signaling pathways via phosphorylation of both Akt/protein kinase B and p27Kip1 in neuroblastoma. Cancer Res 68, 9825-31 19047162
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T308-p - Akt1 (human)
Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  LAN-1 (neural crest)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sardomozide no change compared to control

S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  LAN-1 (neural crest)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
eflornithine increase
sardomozide eflornithine no effect upon treatment-induced increase
putrescine eflornithine inhibit treatment-induced increase
spermine eflornithine inhibit treatment-induced increase
spermidine eflornithine inhibit treatment-induced increase
sardomozide no change compared to control
eflornithine increase
A-443654 no change compared to control
LY294002 no change compared to control
LY294002 eflornithine inhibit treatment-induced increase
A-443654 eflornithine inhibit treatment-induced increase
spermidine eflornithine inhibit treatment-induced increase

S256-p - FOXO1A (human)
Modsite: sPrRrAAsMDNNskF SwissProt Entrez-Gene
Orthologous residues
FOXO1A (human): S256‑p, FOXO1A (mouse): S253‑p, FOXO1A (rat): S250‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  LAN-1 (neural crest)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sardomozide no change compared to control

S9-p - GSK3B (human)
Modsite: SGRPRttsFAEsCkP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  LAN-1 (neural crest)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
eflornithine increase
sardomozide eflornithine no effect upon treatment-induced increase
putrescine eflornithine inhibit treatment-induced increase
spermine eflornithine inhibit treatment-induced increase
spermidine eflornithine inhibit treatment-induced increase
LY294002 no change compared to control
A-443654 no change compared to control
eflornithine increase
LY294002 eflornithine no effect upon treatment-induced increase
A-443654 eflornithine no effect upon treatment-induced increase
spermidine eflornithine no effect upon treatment-induced increase

S10-p - p27Kip1 (human)
Modsite: NVRVSNGsPsLErMD SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): S10‑p, p27Kip1 (mouse): S10‑p, p27Kip1 (rat): S10‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  LAN-1 (neural crest)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
eflornithine increase
spermidine eflornithine no effect upon treatment-induced increase
LY294002 eflornithine augment treatment-induced increase
LY294002 increase
A-443654 eflornithine augment treatment-induced increase

T187-p - p27Kip1 (human)
Modsite: NAGsVEQtPKKPGLR SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T187‑p, p27Kip1 (mouse): T187‑p, p27Kip1 (rat): T187‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  LAN-1 (neural crest)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
eflornithine no change compared to control
spermidine, eflornithine no change compared to control
LY294002 no change compared to control
LY294002, eflornithine no change compared to control
A-443654, eflornithine no change compared to control
A-443654 no change compared to control

T198-p - p27Kip1 (human)
Modsite: PGLRRRQt_______ SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T198‑p, p27Kip1 (mouse): T197‑p, p27Kip1 (rat): T197‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  LAN-1 (neural crest)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
eflornithine increase
spermidine eflornithine no effect upon treatment-induced increase
LY294002 eflornithine augment treatment-induced increase
LY294002 increase
A-443654 eflornithine augment treatment-induced increase

S241-p - PDK1 (human)
Modsite: skQARANsFVGtAQy SwissProt Entrez-Gene
Orthologous residues
PDK1 (human): S241‑p, PDK1 iso2 (human): S191‑p, PDK1 iso4 (human): S114‑p, PDK1 iso5 (human): S241‑p, PDK1 (mouse): S244‑p, PDK1 (rat): S244‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  LAN-1 (neural crest)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sardomozide no change compared to control

S380-p - PTEN (human)
Modsite: EPDHyRYsDttDsDP SwissProt Entrez-Gene
Orthologous residues
PTEN (human): S380‑p, PTEN iso2 (human): S553‑p, PTEN (mouse): S380‑p, PTEN (rat): S380‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  LAN-1 (neural crest)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sardomozide no change compared to control