Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 18760948
Chuderland D, Konson A, Seger R (2008) Identification and characterization of a general nuclear translocation signal in signaling proteins. Mol Cell 31, 850-61 18760948
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

T202-p - ERK1 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  green monkey
Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase ERK2 wild-type
phorbol_ester phorbol_ester inhibit treatment-induced increase S244-6A mutant

Y204-p - ERK1 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  green monkey
Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase ERK2 wild-type
phorbol_ester phorbol_ester inhibit treatment-induced increase S244-6A mutant

T185-p - ERK2 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  green monkey
Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase ERK2 wild-type
phorbol_ester phorbol_ester inhibit treatment-induced increase S244-6A mutant

Y187-p - ERK2 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  green monkey
Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase ERK2 wild-type
phorbol_ester phorbol_ester inhibit treatment-induced increase S244-6A mutant

S246-p - ERK2 (human)
Modsite: HILGILGsPsQEDLN SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): S246‑p, ERK2 (mouse): S244‑p, ERK2 (rat): S244‑p, ERK2 (chicken): S254‑p, ERK2 (cow): S246‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  green monkey
Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
vanadate increase
phorbol_ester increase
EGF increase
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
IPO7 (human) Induces intracellular localization co-immunoprecipitation
NUP153 (human) Disrupts molecular association, regulation co-immunoprecipitation
Comments:  nuclear translocation

S248-p - ERK2 (human)
Modsite: LGILGsPsQEDLNCI SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): S248‑p, ERK2 (mouse): S246‑p, ERK2 (rat): S246‑p, ERK2 (chicken): S256‑p, ERK2 (cow): S248‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  green monkey
Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
vanadate increase
phorbol_ester increase
EGF increase
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
IPO7 (human) Induces intracellular localization co-immunoprecipitation
NUP153 (human) Disrupts molecular association, regulation co-immunoprecipitation
Comments:  nuclear translocation

T386-p - MEK1 (human)
Modsite: IGLNQPstPtHAAGV SwissProt Entrez-Gene
Orthologous residues
MEK1 (human): T386‑p, MEK1 iso2 (human): T360‑p, MEK1 (mouse): T386‑p, MEK1 (rat): T386‑p, MEK1 (rabbit): T386‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  green monkey
Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  nuclear translocation

T388-p - MEK1 (human)
Modsite: LNQPstPtHAAGV__ SwissProt Entrez-Gene
Orthologous residues
MEK1 (human): T388‑p, MEK1 iso2 (human): T362‑p, MEK1 (mouse): T388‑p, MEK1 (rat): T388‑p, MEK1 (rabbit): T388‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  green monkey
Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  nuclear translocation

S416-p - SMAD3 (human)
Modsite: KVLtQMGsPsIRCss SwissProt Entrez-Gene
Orthologous residues
SMAD3 (human): S416‑p, SMAD3 (mouse): S416‑p, SMAD3 (rat): S416‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  green monkey
Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  nuclear translocation

S418-p - SMAD3 (human)
Modsite: LtQMGsPsIRCssVs SwissProt Entrez-Gene
Orthologous residues
SMAD3 (human): S418‑p, SMAD3 (mouse): S418‑p, SMAD3 (rat): S418‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  green monkey
Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  nuclear translocation