Curated Information
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Home > Curated Information Page > PubMed Id: 19236849
Zschoernig B, Mahlknecht U (2009) Carboxy-terminal phosphorylation of SIRT1 by protein kinase CK2. Biochem Biophys Res Commun 381, 372-7 19236849
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S27-p - SIRT1 (human)
Modsite: ADREAAssPAGEPLR SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): S27‑p, SIRT1 (mouse): Q26‑p, SIRT1 (rat): Q26‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

S47-p - SIRT1 (human)
Modsite: DGPGLERsPGEPGGA SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): S47‑p, SIRT1 (mouse): S46‑p, SIRT1 (rat): S46‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

S659-p - SIRT1 (human)
Modsite: FHGAEVYsDsEDDVL SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): S659‑p, SIRT1 (mouse): S649‑p, SIRT1 (rat): S652‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) modification site within consensus motif

S661-p - SIRT1 (human)
Modsite: GAEVYsDsEDDVLSS SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): S661‑p, SIRT1 (mouse): S651‑p, SIRT1 (rat): S654‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) modification site within consensus motif