Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 19217413
Stehmeier P, Muller S (2009) Phospho-regulated SUMO interaction modules connect the SUMO system to CK2 signaling. Mol Cell 33, 400-9 19217413
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S392-p - EXOSC9 (human)
Modsite: QDAPIILsDsEEEEM SwissProt Entrez-Gene
Orthologous residues
EXOSC9 (human): S392‑p, EXOSC9 iso2 (human): S409‑p, EXOSC9 (mouse): S393‑p, EXOSC9 (rat): S392‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  S.cerevisiae
Cellular systems studied:  cell lines
Species studied:  yeast
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TBB decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SUMO2 (human) Induces yeast two-hybrid
SUMO1 (human) Induces yeast two-hybrid

S394-p - EXOSC9 (human)
Modsite: APIILsDsEEEEMII SwissProt Entrez-Gene
Orthologous residues
EXOSC9 (human): S394‑p, EXOSC9 iso2 (human): S411‑p, EXOSC9 (mouse): S395‑p, EXOSC9 (rat): S394‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  S.cerevisiae
Cellular systems studied:  cell lines
Species studied:  yeast
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TBB decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SUMO2 (human) Induces yeast two-hybrid
SUMO1 (human) Induces yeast two-hybrid

S466-p - PIAS1 (human)
Modsite: VIDLtIDsssDEEEE SwissProt Entrez-Gene
Orthologous residues
PIAS1 (human): S466‑p, PIAS1 iso2 (human): S468‑p, PIAS1 (mouse): S466‑p, PIAS1 (rat): S466‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), S.cerevisiae
Cellular systems studied:  cell lines
Species studied:  human, yeast
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) phospho-motif antibody, transfection of wild-type enzyme, modification site within consensus motif, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
sorbitol TBB inhibit treatment-induced increase
TBB decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SUMO2 (human) Induces yeast two-hybrid, co-immunoprecipitation
SUMO1 (human) Induces yeast two-hybrid, co-immunoprecipitation

S467-p - PIAS1 (human)
Modsite: IDLtIDsssDEEEEE SwissProt Entrez-Gene
Orthologous residues
PIAS1 (human): S467‑p, PIAS1 iso2 (human): S469‑p, PIAS1 (mouse): S467‑p, PIAS1 (rat): S467‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), S.cerevisiae
Cellular systems studied:  cell lines
Species studied:  human, yeast
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) phospho-motif antibody, transfection of wild-type enzyme, modification site within consensus motif, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
sorbitol TBB inhibit treatment-induced increase
TBB decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SUMO2 (human) Induces yeast two-hybrid, co-immunoprecipitation
SUMO1 (human) Induces yeast two-hybrid, co-immunoprecipitation

S468-p - PIAS1 (human)
Modsite: DLtIDsssDEEEEEP SwissProt Entrez-Gene
Orthologous residues
PIAS1 (human): S468‑p, PIAS1 iso2 (human): S470‑p, PIAS1 (mouse): S468‑p, PIAS1 (rat): S468‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), S.cerevisiae
Cellular systems studied:  cell lines
Species studied:  human, yeast
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) phospho-motif antibody, transfection of wild-type enzyme, modification site within consensus motif, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
sorbitol TBB inhibit treatment-induced increase
TBB decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SUMO1 (human) Induces yeast two-hybrid, co-immunoprecipitation
SUMO2 (human) Induces yeast two-hybrid, co-immunoprecipitation

S560-p - PML (human)
Modsite: EERVVVIsssEDsDA SwissProt Entrez-Gene
Orthologous residues
PML (human): S560‑p, PML iso2 (human): S560‑p, PML iso3 (human): S560‑p, PML iso4 (human): , PML iso5 (human): S560‑p, PML iso8 (human): S560‑p, PML iso10 (human): , PML iso11 (human): S512‑p, PML iso12 (human): S512‑p, PML iso14 (human): , PML (mouse): S570‑p, PML iso2 (mouse): S524‑p, PML (rat): S558‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  S.cerevisiae
Cellular systems studied:  cell lines
Species studied:  yeast
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SUMO1 (human) Induces yeast two-hybrid

S561-p - PML (human)
Modsite: ERVVVIsssEDsDAE SwissProt Entrez-Gene
Orthologous residues
PML (human): S561‑p, PML iso2 (human): S561‑p, PML iso3 (human): S561‑p, PML iso4 (human): , PML iso5 (human): S561‑p, PML iso8 (human): S561‑p, PML iso10 (human): , PML iso11 (human): S513‑p, PML iso12 (human): S513‑p, PML iso14 (human): , PML (mouse): S571‑p, PML iso2 (mouse): S525‑p, PML (rat): S559‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  S.cerevisiae
Cellular systems studied:  cell lines
Species studied:  yeast
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SUMO1 (human) Induces yeast two-hybrid

S562-p - PML (human)
Modsite: RVVVIsssEDsDAEN SwissProt Entrez-Gene
Orthologous residues
PML (human): S562‑p, PML iso2 (human): S562‑p, PML iso3 (human): S562‑p, PML iso4 (human): , PML iso5 (human): S562‑p, PML iso8 (human): S562‑p, PML iso10 (human): , PML iso11 (human): S514‑p, PML iso12 (human): S514‑p, PML iso14 (human): , PML (mouse): S572‑p, PML iso2 (mouse): S526‑p, PML (rat): S560‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  S.cerevisiae
Cellular systems studied:  cell lines
Species studied:  yeast
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SUMO2 (human) Induces yeast two-hybrid, co-immunoprecipitation
SUMO1 (human) Induces yeast two-hybrid, co-immunoprecipitation
SUMO1 (human) Induces yeast two-hybrid

S565-p - PML (human)
Modsite: VIsssEDsDAENSSS SwissProt Entrez-Gene
Orthologous residues
PML (human): S565‑p, PML iso2 (human): S565‑p, PML iso3 (human): S565‑p, PML iso4 (human): , PML iso5 (human): S565‑p, PML iso8 (human): S565‑p, PML iso10 (human): , PML iso11 (human): S517‑p, PML iso12 (human): S517‑p, PML iso14 (human): , PML (mouse): S575‑p, PML iso2 (mouse): S529‑p, PML (rat): S563‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  S.cerevisiae
Cellular systems studied:  cell lines
Species studied:  yeast
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)