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Home > Curated Information Page > PubMed Id: 18952597
Hu D, et al. (2008) c-Jun N-terminal kinase 1 interacts with and negatively regulates Wnt/beta-catenin signaling through GSK3beta pathway. Carcinogenesis 29, 2317-24 18952597
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S33-p - CTNNB1 (mouse)
Modsite: QQQsYLDsGIHsGAT SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): S33‑p, CTNNB1 (mouse): S33‑p, CTNNB1 (rat): S33‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast) [JNK1 (mouse), transfection]
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse

S37-p - CTNNB1 (mouse)
Modsite: YLDsGIHsGATtTAP SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): S37‑p, CTNNB1 (mouse): S37‑p, CTNNB1 (rat): S37‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast) [JNK1 (mouse), transfection]
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse

S9-p - GSK3B (mouse)
Modsite: SGRPRttsFAEsCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast) [JNK1 (mouse), transfection]
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CTNNB1 (mouse) increase
CTNNB1 (mouse), JNK1 (human) inhibit treatment-induced increase
lithium CTNNB1 (mouse) augment treatment-induced increase
lithium CTNNB1 (mouse), JNK1 (human) no effect upon treatment-induced increase
UV decrease
MG132 CTNNB1 (human), JNK1 (human) inhibit treatment-induced decrease

T183-p - JNK1 (mouse)
Modsite: AGtsFMMtPyVVtRY SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast) [JNK1 (mouse), transfection]
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
JNK1 (human) increase constitutively active JNK1
CTNNB1 (mouse) decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation, protein degradation
Effect of modification (process):  transcription, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
GSK3B (human) Induces co-immunoprecipitation
CTNNB1 (human) Induces co-immunoprecipitation
Comments:  degredation of beta-catenin.

Y185-p - JNK1 (mouse)
Modsite: tsFMMtPyVVtRYYR SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast) [JNK1 (mouse), transfection]
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
JNK1 (human) increase constitutively active JNK1
CTNNB1 (mouse) decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation, protein degradation
Effect of modification (process):  transcription, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
GSK3B (human) Induces co-immunoprecipitation
CTNNB1 (human) Induces co-immunoprecipitation
Comments:  degredation of beta-catenin.

S63-p - Jun (mouse)
Modsite: kNsDLLtsPDVGLLK SwissProt Entrez-Gene
Orthologous residues
Jun (human): S63‑p, Jun (mouse): S63‑p, Jun (rat): S63‑p, Jun (cow): S63‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast) [JNK1 (mouse), transfection]
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
JNK1 (human) increase constitutively active JNK1
CTNNB1 (mouse) decrease

S73-p - Jun (mouse)
Modsite: VGLLKLAsPELERLI SwissProt Entrez-Gene
Orthologous residues
Jun (human): S73‑p, Jun (mouse): S73‑p, Jun (rat): S73‑p, Jun (cow): S73‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast) [JNK1 (mouse), transfection]
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
JNK1 (human) increase constitutively active JNK1
CTNNB1 (mouse) decrease