Curated Information
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Home > Curated Information Page > PubMed Id: 19218462
McAvoy T, Zhou MM, Greengard P, Nairn AC (2009) Phosphorylation of Rap1GAP, a striatally enriched protein, by protein kinase A controls Rap1 activity and dendritic spine morphology. Proc Natl Acad Sci U S A 106, 3531-6 19218462
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S441-p - RAP1GAP (human)
Modsite: VIRsRsQsMDAMGLS SwissProt Entrez-Gene
Orthologous residues
RAP1GAP (human): S441‑p, RAP1GAP iso2 (human): S441‑p, RAP1GAP iso3 (human): S471‑p, RAP1GAP iso4 (human): S505‑p, RAP1GAP (mouse): S441‑p, RAP1GAP iso2 (mouse): S505‑p, RAP1GAP iso3 (mouse): S472‑p, RAP1GAP iso4 (mouse): S441‑p, RAP1GAP iso6 (mouse): S441‑p, RAP1GAP (rat): S472‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, phosphopeptide mapping
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  293 (epithelial), Neuro-2a (neuron)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Downstream Regulation
Effect of modification (function):  activity, induced
Comments:  increased Rap1 activity

S499-p - RAP1GAP (human)
Modsite: PFGsRRssAIGIENI SwissProt Entrez-Gene
Orthologous residues
RAP1GAP (human): S499‑p, RAP1GAP iso2 (human): S525‑p, RAP1GAP iso3 (human): S529‑p, RAP1GAP iso4 (human): S563‑p, RAP1GAP (mouse): S499‑p, RAP1GAP iso2 (mouse): S563‑p, RAP1GAP iso3 (mouse): S530‑p, RAP1GAP iso4 (mouse): S499‑p, RAP1GAP iso6 (mouse): S525‑p, RAP1GAP (rat): S530‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, phosphopeptide mapping
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  293 (epithelial), Neuro-2a (neuron)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Downstream Regulation
Effect of modification (function):  activity, induced
Comments:  increased Rap1 activity

S441-p - RAP1GAP (mouse)
Modsite: VIRsRSQsMDAMGLS SwissProt Entrez-Gene
Orthologous residues
RAP1GAP (human): S441‑p, RAP1GAP iso2 (human): S441‑p, RAP1GAP iso3 (human): S471‑p, RAP1GAP iso4 (human): S505‑p, RAP1GAP (mouse): S441‑p, RAP1GAP iso2 (mouse): S505‑p, RAP1GAP iso3 (mouse): S472‑p, RAP1GAP iso4 (mouse): S441‑p, RAP1GAP iso6 (mouse): S441‑p, RAP1GAP (rat): S472‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, striatal'-brain
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SKF38393 increase
colforsin increase
NMDA colforsin inhibit treatment-induced increase

S499-p - RAP1GAP (mouse)
Modsite: PFGsRRssAIGIENI SwissProt Entrez-Gene
Orthologous residues
RAP1GAP (human): S499‑p, RAP1GAP iso2 (human): S525‑p, RAP1GAP iso3 (human): S529‑p, RAP1GAP iso4 (human): S563‑p, RAP1GAP (mouse): S499‑p, RAP1GAP iso2 (mouse): S563‑p, RAP1GAP iso3 (mouse): S530‑p, RAP1GAP iso4 (mouse): S499‑p, RAP1GAP iso6 (mouse): S525‑p, RAP1GAP (rat): S530‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, striatal'-brain
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SKF38393 increase
NMDA decrease
colforsin increase
NMDA colforsin inhibit treatment-induced increase
EGTA no change compared to control
NMDA EGTA no change compared to control
ciclosporin no change compared to control
NMDA ciclosporin no change compared to control
okadaic_acid increase
NMDA okadaic_acid inhibit treatment-induced increase

S472-p - RAP1GAP (rat)
Modsite: VIRSRSQsMDAMGLS SwissProt Entrez-Gene
Orthologous residues
RAP1GAP (human): S441‑p, RAP1GAP iso2 (human): S441‑p, RAP1GAP iso3 (human): S471‑p, RAP1GAP iso4 (human): S505‑p, RAP1GAP (mouse): S441‑p, RAP1GAP iso2 (mouse): S505‑p, RAP1GAP iso3 (mouse): S472‑p, RAP1GAP iso4 (mouse): S441‑p, RAP1GAP iso6 (mouse): S441‑p, RAP1GAP (rat): S472‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, hippocampal'-'brain, embryonic'
Cellular systems studied:  primary cultured cells
Species studied:  rat

S530-p - RAP1GAP (rat)
Modsite: PFGSRRSsAIGIENI SwissProt Entrez-Gene
Orthologous residues
RAP1GAP (human): S499‑p, RAP1GAP iso2 (human): S525‑p, RAP1GAP iso3 (human): S529‑p, RAP1GAP iso4 (human): S563‑p, RAP1GAP (mouse): S499‑p, RAP1GAP iso2 (mouse): S563‑p, RAP1GAP iso3 (mouse): S530‑p, RAP1GAP iso4 (mouse): S499‑p, RAP1GAP iso6 (mouse): S525‑p, RAP1GAP (rat): S530‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, hippocampal'-'brain, embryonic'
Cellular systems studied:  primary cultured cells
Species studied:  rat