Sun Z, Chin YE, Zhang DD (2009) Acetylation of Nrf2 by p300/CBP augments promoter-specific DNA binding of Nrf2 during the antioxidant response. Mol Cell Biol 29, 2658-72 19273602

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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K438-ac - NRF2 (human) ▲

Modsite: PVsPGHrktPFTkDk SwissProt Entrez-Gene Orthologous residues NRF2 (human): K438‑ac, NRF2 (mouse): K430‑ac, NRF2 (rat): K437‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), MDA-MB-231 (breast cell), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

K443-ac - NRF2 (human) ▲

Modsite: HrktPFTkDkHsSRL SwissProt Entrez-Gene Orthologous residues NRF2 (human): K443‑ac, NRF2 (mouse): K435‑ac, NRF2 (rat): K442‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), MDA-MB-231 (breast cell), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

K445-ac - NRF2 (human) ▲

Modsite: ktPFTkDkHsSRLEA SwissProt Entrez-Gene Orthologous residues NRF2 (human): K445‑ac, NRF2 (mouse): K437‑ac, NRF2 (rat): K444‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), MDA-MB-231 (breast cell), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

K462-ac - NRF2 (human) ▲

Modsite: TRDELRAkALHIPFP SwissProt Entrez-Gene Orthologous residues NRF2 (human): K462‑ac, NRF2 (mouse): K454‑ac, NRF2 (rat): K461‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), MDA-MB-231 (breast cell), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

K472-ac - NRF2 (human) ▲

Modsite: HIPFPVEkIINLPVV SwissProt Entrez-Gene Orthologous residues NRF2 (human): K472‑ac, NRF2 (mouse): K464‑ac, NRF2 (rat): K471‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), MDA-MB-231 (breast cell), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

K487-ac - NRF2 (human) ▲

Modsite: DFNEMMSkEQFNEAQ SwissProt Entrez-Gene Orthologous residues NRF2 (human): K487‑ac, NRF2 (mouse): K479‑ac, NRF2 (rat): K486‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), MDA-MB-231 (breast cell), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

K506-ac - NRF2 (human) ▲

Modsite: RDIRRRGkNkVAAQN SwissProt Entrez-Gene Orthologous residues NRF2 (human): K506‑ac, NRF2 (mouse): K498‑ac, NRF2 (rat): K505‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), MDA-MB-231 (breast cell), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

K508-ac - NRF2 (human) ▲

Modsite: IRRRGkNkVAAQNCR SwissProt Entrez-Gene Orthologous residues NRF2 (human): K508‑ac, NRF2 (mouse): K500‑ac, NRF2 (rat): K507‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), MDA-MB-231 (breast cell), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

K516-ac - NRF2 (human) ▲

Modsite: VAAQNCRkRkLENIV SwissProt Entrez-Gene Orthologous residues NRF2 (human): K516‑ac, NRF2 (mouse): K508‑ac, NRF2 (rat): K515‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), MDA-MB-231 (breast cell), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

K518-ac - NRF2 (human) ▲

Modsite: AQNCRkRkLENIVEL SwissProt Entrez-Gene Orthologous residues NRF2 (human): K518‑ac, NRF2 (mouse): K510‑ac, NRF2 (rat): K517‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), MDA-MB-231 (breast cell), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

K533-ac - NRF2 (human) ▲

Modsite: EQDLDHLkDEkEkLL SwissProt Entrez-Gene Orthologous residues NRF2 (human): K533‑ac, NRF2 (mouse): K525‑ac, NRF2 (rat): K532‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), MDA-MB-231 (breast cell), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

K536-ac - NRF2 (human) ▲

Modsite: LDHLkDEkEkLLkEk SwissProt Entrez-Gene Orthologous residues NRF2 (human): K536‑ac, NRF2 (mouse): R528‑ac, NRF2 (rat): R535‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), MDA-MB-231 (breast cell), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

K538-ac - NRF2 (human) ▲

Modsite: HLkDEkEkLLkEkGE SwissProt Entrez-Gene Orthologous residues NRF2 (human): K538‑ac, NRF2 (mouse): K530‑ac, NRF2 (rat): K537‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), MDA-MB-231 (breast cell), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

K541-ac - NRF2 (human) ▲

Modsite: DEkEkLLkEkGENDk SwissProt Entrez-Gene Orthologous residues NRF2 (human): K541‑ac, NRF2 (mouse): R533‑ac, NRF2 (rat): R540‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), MDA-MB-231 (breast cell), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

K543-ac - NRF2 (human) ▲

Modsite: kEkLLkEkGENDkSL SwissProt Entrez-Gene Orthologous residues NRF2 (human): K543‑ac, NRF2 (mouse): K535‑ac, NRF2 (rat): K542‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), MDA-MB-231 (breast cell), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

K548-ac - NRF2 (human) ▲

Modsite: kEkGENDkSLHLLkk SwissProt Entrez-Gene Orthologous residues NRF2 (human): K548‑ac, NRF2 (mouse): R540‑ac, NRF2 (rat): R547‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), MDA-MB-231 (breast cell), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

K554-ac - NRF2 (human) ▲

Modsite: DkSLHLLkkQLstLy SwissProt Entrez-Gene Orthologous residues NRF2 (human): K554‑ac, NRF2 (mouse): K546‑ac, NRF2 (rat): K553‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), MDA-MB-231 (breast cell), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

K555-ac - NRF2 (human) ▲

Modsite: kSLHLLkkQLstLyL SwissProt Entrez-Gene Orthologous residues NRF2 (human): K555‑ac, NRF2 (mouse): R547‑ac, NRF2 (rat): R554‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), MDA-MB-231 (breast cell), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE CBP (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces