Curated Information
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Home > Curated Information Page > PubMed Id: 16055726
Kulikov R, Boehme KA, Blattner C (2005) Glycogen synthase kinase 3-dependent phosphorylation of Mdm2 regulates p53 abundance. Mol Cell Biol 25, 7170-80 16055726
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S9-p - GSK3B (mouse)
Modsite: SGRPRttsFAEsCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], COS (fibroblast), U2OS (bone cell) [GR (human)]
Cellular systems studied:  cell lines
Species studied:  green monkey, human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, inhibited

S240-p - MDM2 (mouse)
Modsite: CLDQDsVsDQFsVEF SwissProt Entrez-Gene
Orthologous residues
MDM2 (human): S242‑p, MDM2 (mouse): S240‑p, MDM2 (rat): S210‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], COS (fibroblast), U2OS (bone cell) [GR (human)]
Cellular systems studied:  cell lines
Species studied:  green monkey, human, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (mouse) microscopy-colocalization, co-immunoprecipitation

S244-p - MDM2 (mouse)
Modsite: DsVsDQFsVEFEVES SwissProt Entrez-Gene
Orthologous residues
MDM2 (human): S246‑p, MDM2 (mouse): S244‑p, MDM2 (rat): S214‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], COS (fibroblast), U2OS (bone cell) [GR (human)]
Cellular systems studied:  cell lines
Species studied:  green monkey, human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK1D (human)

S254-p - MDM2 (mouse)
Modsite: FEVESLDsEDYsLSD SwissProt Entrez-Gene
Orthologous residues
MDM2 (human): S256‑p, MDM2 (mouse): S254‑p, MDM2 (rat): S224‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], COS (fibroblast), U2OS (bone cell) [GR (human)]
Cellular systems studied:  cell lines
Species studied:  green monkey, human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3B (mouse)