Curated Information
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Home > Curated Information Page > PubMed Id: 16081872
Partovian C, et al. (2005) PKCalpha activates eNOS and increases arterial blood flow in vivo. Circ Res 97, 482-7 16081872
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S473-p - Akt1 (mouse)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  BAEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FGF2 increase

T494-p - eNOS (mouse)
Modsite: AGITRKKtFKEVANA SwissProt Entrez-Gene
Orthologous residues
eNOS (human): T495‑p, eNOS (mouse): T494‑p, eNOS (rat): T494‑p, eNOS (rabbit): T501‑p, eNOS (pig): T497‑p, eNOS (cow): T497‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, phospho-antibody
Relevant cell lines - cell types - tissues:  BAEC (endothelial), HUVEC (endothelial), myocyte-heart
Cellular systems studied:  cell lines, primary cells
Species studied:  cow, human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FGF2 no change compared to control

S1176-p - eNOS (mouse)
Modsite: TSRIRtQsFsLQERQ SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S1177‑p, eNOS (mouse): S1176‑p, eNOS (rat): S1176‑p, eNOS (rabbit): S1183‑p, eNOS (pig): S1179‑p, eNOS (cow): S1179‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, phospho-antibody
Relevant cell lines - cell types - tissues:  BAEC (endothelial), HUVEC (endothelial), myocyte-heart
Cellular systems studied:  cell lines, primary cells
Species studied:  cow, human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FGF2 increase
PKCA (mouse) increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced