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Home > Curated Information Page > PubMed Id: 18497757
Encinas M, et al. (2008) Analysis of Ret knockin mice reveals a critical role for IKKs, but not PI 3-K, in neurotrophic factor-induced survival of sympathetic neurons. Cell Death Differ 15, 1510-21 18497757
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y981-p - Ret (human)
Modsite: DNCSEEMyRLMLQCW SwissProt Entrez-Gene
Orthologous residues
Ret (human): Y981‑p, Ret iso2 (human): Y981‑p, Ret iso3 (human): Y505‑p, Ret (mouse): Y982‑p, Ret iso2 (mouse): Y982‑p, Ret iso4 (mouse): , Ret (rat): Y982‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  'neuron, superior cervical ganglion'-brain [Ret (mouse), homozygous knockout]
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Comments:  Ret9 or Ret51 knock-in

Y1015-p - Ret (human)
Modsite: MMVKRRDyLDLAAST SwissProt Entrez-Gene
Orthologous residues
Ret (human): Y1015‑p, Ret iso2 (human): Y1015‑p, Ret iso3 (human): Y539‑p, Ret (mouse): Y1016‑p, Ret iso2 (mouse): Y1016‑p, Ret iso4 (mouse): , Ret (rat): Y1016‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  'neuron, superior cervical ganglion'-brain [Ret (mouse), homozygous knockout]
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Comments:  Ret9 or Ret51 knock-in

Y1062-p - Ret (human)
Modsite: TWIENkLyGMsDPNW SwissProt Entrez-Gene
Orthologous residues
Ret (human): Y1062‑p, Ret iso2 (human): Y1062‑p, Ret iso3 (human): Y586‑p, Ret (mouse): Y1063‑p, Ret iso2 (mouse): Y1063‑p, Ret iso4 (mouse): , Ret (rat): Y1063‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  'neuron, superior cervical ganglion'-brain [Ret (mouse), homozygous knockout]
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Comments:  Ret9 or Ret51 knock-in

T308-p - Akt1 (mouse)
Modsite: KDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, superior cervical ganglion'
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
siRNA PDK1 (mouse) inhibit treatment-induced increase
GDNF increase
U0126 GDNF inhibit treatment-induced increase

S473-p - Akt1 (mouse)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, superior cervical ganglion'
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GDNF Ret (human) increase Ret51, 9 knockin
GDNF increase Ret wild-type
GDNF GDNF inhibit treatment-induced increase Ret 9 Y981 mutant, slight increase compared to untreated
GDNF GDNF inhibit treatment-induced increase Ret9 Y1015F mutant; sl
GDNF GDNF inhibit treatment-induced increase Ret9 Y1062F mutant
GDNF increase
LY294002 GDNF inhibit treatment-induced increase

T203-p - ERK1 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, superior cervical ganglion'
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GDNF Ret (human) increase Ret51, 9 knockin
GDNF increase Ret wild-type
GDNF GDNF inhibit treatment-induced increase Ret 9 Y981 mutant, slight increase compared to untreated
GDNF GDNF inhibit treatment-induced increase Ret9 Y1015F mutant; sl
GDNF GDNF inhibit treatment-induced increase Ret9 Y1062F mutant
GDNF increase
U0126 GDNF inhibit treatment-induced increase
BRAF (human) increase constitutively active B-Raf
GDNF no effect upon treatment-induced increase

Y205-p - ERK1 (mouse)
Modsite: HtGFLtEyVAtRWyR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, superior cervical ganglion'
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GDNF Ret (human) increase Ret51, 9 knockin
GDNF increase Ret wild-type
GDNF GDNF inhibit treatment-induced increase Ret 9 Y981 mutant, slight increase compared to untreated
GDNF GDNF inhibit treatment-induced increase Ret9 Y1015F mutant; sl
GDNF GDNF inhibit treatment-induced increase Ret9 Y1062F mutant
GDNF increase
U0126 GDNF inhibit treatment-induced increase
BRAF (human) increase constitutively active B-Raf
GDNF no effect upon treatment-induced increase

T183-p - ERK2 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, superior cervical ganglion'
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GDNF Ret (human) increase Ret51, 9 knockin
GDNF increase Ret wild-type
GDNF GDNF inhibit treatment-induced increase Ret 9 Y981 mutant, slight increase compared to untreated
GDNF GDNF inhibit treatment-induced increase Ret9 Y1015F mutant; sl
GDNF GDNF inhibit treatment-induced increase Ret9 Y1062F mutant
GDNF increase
U0126 GDNF inhibit treatment-induced increase
BRAF (human) increase constitutively active B-Raf
GDNF no effect upon treatment-induced increase

Y185-p - ERK2 (mouse)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, superior cervical ganglion'
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GDNF Ret (human) increase Ret51, 9 knockin
GDNF increase Ret wild-type
GDNF GDNF inhibit treatment-induced increase Ret 9 Y981 mutant, slight increase compared to untreated
GDNF GDNF inhibit treatment-induced increase Ret9 Y1015F mutant; sl
GDNF GDNF inhibit treatment-induced increase Ret9 Y1062F mutant
GDNF increase
U0126 GDNF inhibit treatment-induced increase
BRAF (human) increase constitutively active B-Raf
GDNF no effect upon treatment-induced increase

S180-p - IKKA (mouse)
Modsite: DQGsLCTsFVGTLQY SwissProt Entrez-Gene
Orthologous residues
IKKA (human): S180‑p, IKKA (mouse): S180‑p, IKKA (rat): S180‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, superior cervical ganglion'
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GDNF increase
NGF increase
siRNA GDNF BRAF (human) inhibit treatment-induced increase
siRNA NGF BRAF (human) inhibit treatment-induced increase

S181-p - IKKB (mouse)
Modsite: DQGsLCTsFVGTLQy SwissProt Entrez-Gene
Orthologous residues
IKKB (human): S181‑p, IKKB (mouse): S181‑p, IKKB (rat): S181‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, superior cervical ganglion'
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GDNF increase
NGF increase
siRNA GDNF BRAF (human) inhibit treatment-induced increase
siRNA NGF BRAF (human) inhibit treatment-induced increase